Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells

Authors

  • Rungtip Soi-ampornkul Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Sandeepa Ghimire Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Wipawan Thangnipon Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom 73170
  • Nirut Suwanna Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140
  • Chinnavuth Vatanashevanopakorn Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Keywords:

Curcumin; apoptosis; anti-oxidant; oxidative stress; SK-N-SH cells

Abstract

Objective: Cellular damage induced by oxidative stress has been involved in the development of neurodegeneration. Curcumin, a dietary polyphenol found in the rhizome of Curcuma longa, has been shown, both in vitro and in vivo, to be an effective reactive oxygen scavenging molecule. We investigated an anti-oxidative effect of curcumin against H2O2-induced toxicity in human neuroblastoma cell line SK-N-SH.
Methods: The SK-N-SH cells were pre-treated with curcumin 2 hours prior to H2O2 treatment. We measured cell viability, intracellular reactive oxygen species (ROS) levels, expression of apoptotic-related proteins and caspase-3 activity 24 hours post H2O2-induced cytoxicity.
Results: Treatment with curcumin at concentrations ranging from 5 to 50 μg/mL was not cytotoxic. Pre-treatment with curcumin at the concentrations of 5 to 50 μg/mL prior to H2O2 exposure caused a significant decrease in intracellular ROS levels and a significant increase in cell viability in a dose-dependent manner. Expression of activated form of caspase-3 and BAX, a pro-apoptotic protein, measured by Western blotting were reduced when the SK-NSH cell line was pre-incubated with curcumin. The curcumin pre-treated cells also exhibited less caspase-3 activity.
Conclusion: Curcumin has protective effects against H2O2-induced toxicity in a dose-dependent manner through its anti-apoptotic and anti-oxidative properties in an in vitro H2O2-treated SK-N-SH model.

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Published

19-06-2018

How to Cite

Soi-ampornkul, R., Ghimire, S., Thangnipon, W., Suwanna, N., & Vatanashevanopakorn, C. (2018). Curcumin Attenuates Hydrogen Peroxide-Induced Cytotoxicity in Human Neuroblastoma SK-N-SH Cells. Siriraj Medical Journal, 70(3), 184–190. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/129563

Issue

Vol. 70 No. 3 (2018): May-June

Section

Original Article

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