Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia

Pitak Laokirkkiat; Nida Jareemit; Isarin Thanaboonyawat; Hollie Smith; Sukanya Sriiam

doi:10.33192/Smj.2019.02

Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia

Authors

Pitak Laokirkkiat Infertility unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Nida Jareemit Infertility unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Isarin Thanaboonyawat Infertility unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand https://orcid.org/0000-0002-3912-1382
Hollie Smith Nurture Fertility, The East Midlands Fertility Clinic, Nottingham, United Kingdom.
Sukanya Sriiam Infertility unit, Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand

DOI:

https://doi.org/10.33192/Smj.2019.02

Keywords:

Hypoosmotic swelling test; TUNEL; asthenozoospermia; male infertility; intracytoplasmic sperm injection

Abstract

Objective: To study the correlation between the hypoosmotic swelling test (HOST) and DNA fragmentation in
asthenozoospermia assessed by the terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphatebiotin
nick end‐labeling (TUNEL) assay.
Methods: This cross-sectional study was conducted in 27 semen samples obtained from infertile men with
asthenozoospermia. Both HOST and TUNEL assay were performed for each sample. The sperm swelling pattern
and positive apoptosis staining of individual spermatozoa were evaluated. HOST and TUNEL scores, and the
proportion of positive staining in each grade were calculated in each sample.
Results: The results showed a negative correlation between HOST and TUNEL scores (r = -0.428, P = 0.026). Sperm
swelling grade A had a higher incidence of positive apoptosis staining when compared with other grades (P < 0.01).
There was no statistically significant difference in positive apoptotic staining between other grades; nevertheless,
sperm swelling grade D tended to have a lower incidence of positive apoptosis staining.
Conclusion: Based on the results of this study, HOST may be used as an optional test to identify DNA-intact
spermatozoa whereby sperm with a grade D swelling pattern should be selected preferentially for intracytoplasmic
sperm injection (ICSI), whereas sperm with a grade A swelling pattern should be avoided for ICSI.

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Published

15-02-2019

How to Cite

Laokirkkiat, P., Jareemit, N., Thanaboonyawat, I., Smith, H., & Sriiam, S. (2019). Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia. Siriraj Medical Journal, 71(1), 8–13. https://doi.org/10.33192/Smj.2019.02

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Issue

Vol. 71 No. 1 (2019): January-February

Section

Original Article

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