Increase in Endothelin-1 Expression in Umbilical Cord Arteries in Preeclampsia
DOI:
https://doi.org/10.33192/Smj.2020.22Keywords:
Endothelin1; preeclampsia; pregnancy; umbilical cordAbstract
Objective: Endothelin1 (ET1) is 21- amino acid vasoconstrictor peptide secreted by endotheliumwhich has an important role in the pathoohysiology of preeclampsia (PE). The objective of this study was to evaluate the binding sites and quantitative changes in ET1 in umbilical cord vessels of PE patients.
Methods: This study recruited 40 pregnant women between 20-40 years old at 3rd trimester. All cases selected for this study underwent an elective cesarean section, grouped into 2 groups; PE group of 20 pregnant women (at 3rd trimester) who proved to have pregnancy induced hypertension and proteinuria. The control group was of 20 healthy pregnant females at the same average of gestational age and with the same exclusion criteria and no PE, underwent elective caesarean section. Umbilical cord tissues were taken from the maternal side, fixed with formalin, paraffin, embedded sections of umbilical cord were treated with Endothelin1 antibody. The immunoreactivity of ET1 was assessed using Aperio image scope software. Statistical analysis was done using SPSS program.
Results: The results demonstrated a significant increase (P = 0.001) of ET1 expression in cord vessels of PE group with respect to control group (mean 28.5±1.7, 2.6±0.4 respectively).
Conclusion: It is concluded that ET1 is markedly increase in PE and may be the cause behind promoted vascular smooth muscle cell contraction and blood pressure elevation in PE.
References
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34. Fiore G, Florio P, Micheli L, Nencini C, Rossi M, Cerretani D, et al. Endothelin-1 triggers placental oxidative stress pathways: putative role in preeclampsia. J Clin Endocrinol Metab 2005;90:4205-10.
2. Kim DW, Staples M, Shinozuka K, Pantcheva P, Kang SD, Borlongan CV. Wharton’s jelly-derived mesenchymal stem cells: phenotypic characterization and optimizing their therapeutic potential for clinical applications. Int J Mol Sci 2013;14:11692-712.
3. Benirschke K, Kaufmann P, Baergen R. Pathology of the human placenta. 5th ed. New York: Springer; 2006.
4. Barnwal M, Rathi SK, Chhabra S, Nanda S. Histomorphometry of umbilical cord and its vessels in pre-eclampsia as compared to normal pregnancies. NJOG 2012;7:28-32.
5. Sánchez-Aranguren LC, Prada CE, Riaño-Medina CE, Lopez M. Endothelial dysfunction and preeclampsia: role of oxidative stress. Front Physiol 2014;5:372.
6. Uzan J, Carbonnel M, Piconne O, Asmar R, Ayoubi JM. Pre-eclampsia: pathophysiology, diagnosis and management. Vascular Health Risk Manag 2011;7:467-74.
7. Jeyabalan A. Epidemiology of preeclampsia: impact of obesity. Nutr Rev 2013;71:S18-25.
8. Bernardi FC, Vuolo F, Petronilho F, Michels M, Ritter C, Dal-Pizzol F. Plasma nitric oxide, endothelin-1, arginase and superoxide dismutase in the plasma and placentae from preeclamptic patients. An Acad Bras Cienc 2015;87:713-9.
9. Davenport AP, Hyndman KA, Dhaun N, Southan C, Kohan DE, Pollock JS, et al. Endothelin. Pharmacol Rev 2016;68:357-418.
10. Dong F, Zhang X, Wold LE, Ren Q, Zhang Z, Ren J. Endothelin-1 enhances oxidative stress, cell proliferation and reduces apoptosis in human umbilical vein endothelial cells: role of ETB receptor, NADPH oxidase and caveolin-1. Br J Pharmacol 2005;145:323-33.
11. Kowalczyk A, Kleniewska P, Kolodziejczyk M, Skibska B, Goraca A. The role of endothelin-1 and endothelin receptor antagonists in inflammatory response and sepsis. Arch Immunol Ther Exp (Warsz) 2015;63:41-52.
12. Vignon-Zellweger N, Heiden S, Miyauchi T, Emoto N. Endothelin and endothelin receptors in the renal and cardiovascular systems. Life Sci 2012;91:490- 500.
13. Saleh L, Verdonk K, Visser W, van den Meiracker AH, Danser AH. The emerging role of endothelin-1 in the pathogenesis of pre-eclampsia. Ther Adv Cardiovasc Dis 2016;10:282-93.
14. Hemsén A, Gillis C, Larsson O, Haegerstrand A, Lundberg JM. Characterization, localization and actions of endothelins in umbilical vessels and placenta of man. Acta Physiol Scand 1991;143:395-404.
15. Pennington KA, Schlitt JM, Jackson DL, Schulz LC, Schust DJ. Preeclampsia: multiple approaches for a multifactorial disease. Dis Model Mech 2012;5:9-18.
16. Phipps E, Prasanna D, Brima W, Jim B. Preeclampsia: updates in pathogenesis, definitions, and guidelines. Clin J Am Soc Nephrol 2016;11:1102-13.
17. Fisher SJ. Why is placentation abnormal in preeclampsia? Am J Obstet Gynecol 2015;213(4 Suppl):S115-22.
18. Granger JP, Alexander BT, Llinas MT, Bennett WA, Khalil RA. Pathophysiology of preeclmpsia: linking placental ischeia/hypoxia with microvascular dysfunction. Microcirculation 2002;9:147-60.
19. LaMarca BD, Gilbert J, Granger JP. Recent progress toward the understanding of the hypertension during preeclampsia. Hypertension 2008;51:982-8.
20. Raio L, Ghezzi F, Di Naro E, Franchi M, Bolla D, Schneider H. Altered sonographic umbilical cord morphometry in early preeclampsia. Obstet Gynecol 2002;100:311-6.
21. Rajendran P, Rengarajan T, Thangavel J, Nishigaki Y, Sakthisekaran D, Sethi G, et al. The vascular endothelium and human diseases. Int J Biol Sci 2013;9:1057-69.
22. Jain A. Endothelin-1: a key pathological factor in pre-eclampsia? Reprod Biomed Online 2012;25:443-9.
23. Bakrania B, Duncan J, Warrington JP, Granger JP. The Endothelin type a receptor as a potential therapeutic target in preeclampsia. Int J Mol Sci 2017;18:E522.
24. Ihara Y, Sagawa N, Hasegawa M, Okagaki A, Li XM, Inamori K, et al. Concentrations of endothelin-1 in maternal and umbilical cord blood at various stages of pregnancy. J Cardiovasc Pharmacol 1991;17 Suppl 7:S443-5.
25. Kumar V, Abbas AK, Aster JC. Robbins Basic Pathology, 9th ed, 2013. ISBN: 9781437717815.
26. Häkkinen LM, Vuolteenaho OJ, Leppäluoto JP, Laatikainen TJ. Endothelin in maternal and umbilical cord blood in spontaneous labor and at elective cesarean delivery. Obstet Gynecol 1992;80:72-5.
27. Myatt L, Rosenfield RB, Eis AL, Brockman DE, Greer I, Lyall F. Nitrotyrosine residues in placenta. Evidence of peroxynitrite formation and action. Hypertension 1996;28:488-93.
28. Freeman BD, Machado FS, Tanowitz HB, Desruisseaux MS. Endothelin-1 and its role in the pathogenesis of infectious diseases. Life Sci 2014;118:110-9.
29. George EM, Palei AC, Granger JP. Endothelin as a final common pathway in the pathophysiology of preeclampsia: therapeutic implications. Curr Opin Nephrol Hypertens 2012;21:157-62.
30. Nishikawa S, Miyamoto A, Yamamoto H, Ohshika H, Kudo R. The relationship between serum nitrate and endothelin-1 concentrations in preeclampsia. Life Sci 2000;67:1447-54.
31. Erdem M, Erdem A, Erdem O, Yildirim G, Memis L, Himmetoğlu O. Immunohistochemical localization of endothelin-1 in human placenta from normal and growth-restricted pregnancies. Pediatr Dev Pathol 2003;6:307-13.
32. Maguire JJ, Davenport AP. Endothelin receptors and their antagonists. Semin Nephrol 2015;35:125-36.
33. Collino F, Bussolati B, Gerbaudo E, Marozio L, Pelissetto S, Benedetto C, et al. Preeclamptic sera induce nephrin shedding from podocytes through endothelin-1 release by endothelial glomerular cells. Am J Physiol Renal Physiol 2008;294:F1185-94.
34. Fiore G, Florio P, Micheli L, Nencini C, Rossi M, Cerretani D, et al. Endothelin-1 triggers placental oxidative stress pathways: putative role in preeclampsia. J Clin Endocrinol Metab 2005;90:4205-10.
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Published
24-02-2020
How to Cite
Algobori, S. A. K. ., Alhabib, M. F. ., Alobaidy, M. T. ., Al-Ani, I. M. ., Matloup, E. I. ., Faisal, G. G. ., Selman, M. O. ., & Suhail, A. M. (2020). Increase in Endothelin-1 Expression in Umbilical Cord Arteries in Preeclampsia. Siriraj Medical Journal, 72(2), 167–173. https://doi.org/10.33192/Smj.2020.22
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