Mutational Analyses of K-ras Exon 2 in Thailand Colorectal Cancer Tissue Samples

Authors

  • Tanawan Kummalue Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Penporn Sujiwatanarat Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Pattara Wongcharoen Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Paisal Parichatikanond Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Jutamanee Ngernmeesri Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Sathien Sukpanichnant Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Keywords:

Mutation, K-ras, exon 2, Thailand and colorectal cancer

Abstract

Objective: The main purpose of this study was to elucidate the genotype of K-ras gene in Thailand colorectal cancer tissue samples, especially in exon 2, which has never before been reported.
Methods: 106 patientsั samples in formalin fixed paraffin embedded tissue blocks were investigated in this study. DNA was extracted and PCR was performed by using primers specific for the K-ras gene at exon 2. Direct sequencing was performed in a Genetic Analyser ABI3130 with specific software.
Results: The mutation of K-ras exon 2 gene in Thailand colorectal cancer samples accounted for 37.7% of the total. The most common mutation found in this series was the G➞A transition which accounted for 70%.
Conclusion: The incidence of K-ras exon 2 mutation in Thailand colorectal cancer samples was remarkedly similar to previous reports.

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Published

31-03-2020

How to Cite

Kummalue, T. ., Sujiwatanarat, P. ., Wongcharoen, P. ., Parichatikanond, P. ., Ngernmeesri, J. ., & Sukpanichnant, S. . (2020). Mutational Analyses of K-ras Exon 2 in Thailand Colorectal Cancer Tissue Samples. Siriraj Medical Journal, 63(1), 4–7. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/240821

Issue

Vol. 63 No. 1 (2011): January-February

Section

Original Article

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