Derris Scandens Benth Extract Radiosensitizes HT-29 Colon Cancer Cells
Keywords:
Radiosensitizer; Derris scandens Benth;radiotherapy; medicinal plant; cancerAbstract
Objective: To evaluate the radiosensitizing potential of Derris scandens extract in a human colon cancer cell model.
Methods: The radiosensitizing effect of D., scandens extract on human colon cancer cells (HT-29) and non-cancerous human retinal epithelial cells (RPE) was determined by cell survival and micronuclei analysis after irradiation. Cell cycle distribution was analyzed by a flow cytometer.
Results: D. scandens extract powerfully increases the radiosensitivity of HT-29 cells, but is less toxic and does not affect the radiosensitivity of RPE cells. Furthermore, the extract was found to reduce radiation-induced G2/M phase arrest in HT-29 cells, but not in RPE cells.
Conclusion: D., scandens extract may have potential as a radiosensitizer for cancer treatment.
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drugs. Anticancer Agents Med Chem. 2009 Jan;9(1):99-108.
2. Girdhani S, Bhosle SM, Thulsidas SA, Kumar A, Mishra KP. Potential
of radiosensitizing agents in cancer chemo-radiotherapy. J Cancer Res
Ther. 2005 Jul-Sep;1(3):129-31.
3. Katz D, Ito E, Liu FF. On the path to seeking novel radiosensitizers.
Int J Radiat Oncol Biol Phys. 2009 Mar 15;73(4):988-96.
4. McGinn CJ, Lawrence TS. Recent advances in the use of radiosensitizing
nucleosides. Semin Radiat Oncol. 2001 Oct;11(4):270-80.
5. Verheij M, Vens C, van Triest B. Novel therapeutics in combination
with radiotherapy to improve cancer treatment: rationale, mechanisms of
action and clinical perspective. Drug Resist Updat. 2010 Feb-Apr;13(1-2):
29-43.
6. Javvadi P, Segan AT, Tuttle SW, Koumenis C. The chemopreventive agent
curcumin is a potent radiosensitizer of human cervical tumor cells via
increased reactive oxygen species production and overactivation of the
mitogen-activated protein kinase pathway. Mol Pharmacol. 2008 May;73(5):
1491-501.
7. Kim KC, Jun HJ, Kim JS, Kim IG. Enhancement of radiation response
with combined Ganoderma lucidum and Duchesnea chrysantha extracts
in human leukemia HL-60 cells. Int J Mol Med. 2008 Apr;21(4):489-98.
8. Sandur SK, Deorukhkar A, Pandey MK, Pabon AM, Shentu S, Guha S,
et al. Curcumin modulates the radiosensitivity of colorectal cancer cells
by suppressing constitutive and inducible NF-kappaB activity. Int J Radiat
Oncol Biol Phys. 2009 Oct 1;75(2):534-42.
9. Shin JI, Shim JH, Kim KH, Choi HS, Kim JW, Lee HG, et al. Sensitization
of the apoptotic effect of gamma-irradiation in genistein-pretreated
CaSki cervical cancer cells. J Microbiol Biotechnol. 2008 Mar;18(3):523-31.
10. Tiangburanatham W, editor. Dictionary of Thai Medicinal Plants. Bangkok:
Prachumtong Printing; 1996.
11. Dhawan BN, Patnaik GK, Rastogi RP, Singh KK, Tandon JS. Screening
of Indian plants for biological activity: part VI. Indian J Exp Biol. 1977
Mar;15(3):208-19.
12. Laupattarakasem P, Houghton PJ, Hoult JR, Itharat A. An evaluation of
the activity related to inflammation of four plants used in Thailand to
treat arthritis. J Ethnopharmacol. 2003 Apr;85(2-3):207-15.
13. Sriwanthana B, Chavalittumrong P. In vitro effect of Derris scandens on
normal lymphocyte proliferation and its activities on natural killer cells in
normals and HIV-1 infected patients. J Ethnopharmacol. 2001 Jun;76(1):
125-9.
14. Mahabusarakam W, Deachathai S, Phongpaichit S, Jansakul C, Taylor
WC. A benzil and isoflavone derivatives from Derris scandens Benth.
Phytochemistry. 2004 Apr;65(8):1185-91.
15. Rao SA, Srinivas PV, Tiwari AK, Vanka UM, Rao RV, Dasari KR, Rao
MJ. Isolation, characterization and chemobiological quantification of alphaglucosidase
enzyme inhibitory and free radical scavenging constituents
from Derris scandens Benth. J Chromatogr B Analyt Technol Biomed
Life Sci. 2007 Aug 15;855(2):166-72.
16. Rukachaisirikul V, Sukpondma Y, Jansakul C, Taylor WC. Isoflavone
glycosides from Derris scandens. Phytochemistry. 2002 Aug;60(8):827-34.
17. Nusse M, Beisker W, Kramer J, Miller BM, Schreiber GA, Viaggi S, et al.
Measurement of micronuclei by flow cytometry. Methods Cell Biol. 1994;
42 Pt B:149-58.
18. Hematulin A, Sagan D, Eckardt-Schupp F, Moertl S. NBS1 is required
for IGF-1 induced cellular proliferation through the Ras/Raf/MEK/ERK
cascade. Cell Signal. 2008 Dec;20(12):2276-85.
19. Bryce SM, Bemis JC, Avlasevich SL, Dertinger SD. In vitro micronucleus
assay scored by flow cytometry provides a comprehensive evaluation of
cytogenetic damage and cytotoxicity. Mutat Res. 2007 Jun 15;630(1-2):
78-91.
20. Kastan MB, Bartek J. Cell-cycle checkpoints and cancer. Nature. 2004
Nov 18;432(7015):316-23.
21. Sancar A, Lindsey-Boltz LA, Unsal-Kacmaz K, Linn S. Molecular mechanisms
of mammalian DNA repair and the DNA damage checkpoints.
Annu Rev Biochem. 2004;73:39-85.
22. Morrison C, Rieder CL. Chromosome damage and progression into and
through mitosis in vertebrates. DNA Repair (Amst). 2004 Aug-Sep;3(8-9):
1133-9.
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Published
03-04-2020
How to Cite
Hematulin, A. ., Ingkaninan, K. ., Limpeanchob, N. ., & Sagan, D. . (2020). Derris Scandens Benth Extract Radiosensitizes HT-29 Colon Cancer Cells. Siriraj Medical Journal, 63(3), 85–88. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/240917
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