Preimplantation Development of the Cleavage Stage Embryos after Laser Assisted Embryo Biopsy
Keywords:
Preimplantation, Cleavage, Embryos, Embryo BiopsyAbstract
This study was carried out to determine the effect of the duration of exposure to the infrared 1.48 mm diode laser, on the developmental potential of cleavage stage embryos. A total of 69 mouse embryos were included in the study. Twenty-two of which (group A) were biopsied using the laser with a longer duration of exposure (600 ms), meanwhile 47 (group B) were biopsied using the same laser with a shorter period (5 ms). The blastocyst formation rate of group B (46/47, 97.8%) was significantly higher than that of group A (12/22, 54.4%). There were no grade 1 blastocysts or hatching in group A. In contrast, 35 of 46 (76.0%) blastocysts in group B were grade 1 and the hatching rate of group B was 84.7% (39/46). In conclusion, the infrared 1.48 mm diode laser may be effective and safe with the cautious application. A long duration of exposure to the laser can adversely affect the developmental potential of the biopsied embryos. The laser system with a shorter duration of exposure, therefore, is recommended for laser-assisted embryo biopsy.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following conditions:
Copyright Transfer
In submitting a manuscript, the authors acknowledge that the work will become the copyrighted property of Siriraj Medical Journal upon publication.
License
Articles are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). This license allows for the sharing of the work for non-commercial purposes with proper attribution to the authors and the journal. However, it does not permit modifications or the creation of derivative works.
Sharing and Access
Authors are encouraged to share their article on their personal or institutional websites and through other non-commercial platforms. Doing so can increase readership and citations.