Preimplantation Development of the Cleavage Stage Embryos after Laser Assisted Embryo Biopsy

Authors

  • Phakphum Phophong Department of Gynecology and Obstetrics, Faculty of Medicine Siriraj Hospita, Mahidol University, Bangkok, Thailand
  • Alpesh Doshi University College London
  • Joyce C Harper University College London

Keywords:

Preimplantation, Cleavage, Embryos, Embryo Biopsy

Abstract

This study was carried out to determine the effect of the duration of exposure to the infrared 1.48 mm diode laser, on the developmental potential of cleavage stage embryos. A total of 69 mouse embryos were included in the study. Twenty-two of which (group A) were biopsied using the laser with a longer duration of exposure (600 ms), meanwhile 47 (group B) were biopsied using the same laser with a shorter period (5 ms). The blastocyst formation rate of group B (46/47, 97.8%) was significantly higher than that of group A (12/22, 54.4%). There were no grade 1 blastocysts or hatching in group A. In contrast, 35 of 46 (76.0%) blastocysts in group B were grade 1 and the hatching rate of group B was 84.7% (39/46). In conclusion, the infrared 1.48 mm diode laser may be effective and safe with the cautious application. A long duration of exposure to the laser can adversely affect the developmental potential of the biopsied embryos. The laser system with a shorter duration of exposure, therefore, is recommended for laser-assisted embryo biopsy.

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Published

11-08-2020

How to Cite

Phophong, P. ., Doshi, A. ., & Harper, J. C. . (2020). Preimplantation Development of the Cleavage Stage Embryos after Laser Assisted Embryo Biopsy. Siriraj Medical Journal, 53(4), 200–206. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/244106

Issue

Vol. 53 No. 4 (2001): April

Section

Original Article

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