Ultrastructural Features of Sidero - apoptotic Hepatocytes in Thalassemia

Authors

  • Kleophant Thakerngpol Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Vanneet Khawcharoenporn Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Kaewkanjana Mangkalanond Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Naree Choosrichom Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Chaiyoot Buawatana Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok
  • Vicha Sookpatdhee Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok
  • Suthat Ducharoen Thalassemia Research Center, Mahidol University
  • Tinrat Stitnimankarn Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700

Keywords:

Ultrastructure, Sidero-apoptosis, Hepatocyte, Thalassemia

Abstract

Liver tissues from 20 patients with thalassemia were investigated by light and electron microscopy, with focus on ultrastructure of sidero-apoptosis. The results of light microscopy were compared. Sidero-apoptosis was found in 82% in β thal/Hb E disease, 67% in thalassemia Hb H, 100% in and β-thal major, respectively. Regardless of the type of hemoglobin, sidero-apoptosis was present in various degrees. The main criteria used for identification of sidero-apoptosis were marked condensation of chromatin against the nuclear membrane, and fragmentation into nuclear fragments of varying sizes and structures into membrane-bounded sidero-apoptotic bodies. These bodies may be phagocytosed by macrophages or neighboring cells or remain free. However, the cell may also shrink into a dense, rounded mass, as a single sidero-apoptotic body. Sidero-apoptoic bodies were similar to acidophilic bodies, or Councilman bodies, commonly found in viral hepatitis. This preliminary report suggested that sidero-apoptosis is a features of human liver pathology in thalassemia and may be due to hepatotoxicity of iron overload. In addition, many factors or other agents-hormone, killer cells, cytokines, various physical, chemical and viral agents should be considered. We propose the term "sidero-apoptosis" to describe the features resembling acidophilic bodies commonly seen in viral hepatitis but with a heavy deposit of iron.

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Published

27-08-2020

How to Cite

Thakerngpol, K. ., Khawcharoenporn, V. ., Mangkalanond, K. ., Choosrichom, N. ., Buawatana, C. ., Sookpatdhee, V. ., Ducharoen, S. ., & Stitnimankarn, T. . (2020). Ultrastructural Features of Sidero - apoptotic Hepatocytes in Thalassemia. Siriraj Medical Journal, 53(8), 502–508. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/244863

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Original Article