The Possible Antigenic Sources of Brugia pahangi

Authors

  • Jantima Roongruangchai Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Kajee Pilakasiri Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Kosol Roongruangchai Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Chaiyaphruk Pilakasiri Department of Narcotics and Toxicology, Armed Force Research Institute of Medical Science (Thai Component)
  • Prasert Sobhon Department of Anatomy, Faculty of Science, Mahidol University

Keywords:

Filaria, Brugia pahangi, Brugia malayi, Wuchereria bancrofti

Abstract

The antigenic sources of adult and the third larval (L3) stages of Brugia pahangi were detected by indirect immunofluorescent technique. Six panels of antisera were used, including human antisera against Brugia malayi and Wuchereria bancrofti, cat antisera against B.malayi and B.pahangi and jird antisera against B.malayi and B.pahangi as primary antibodies. All antisera gave the same results, although four of the six were not infected by B.pahangi. This indicates non-species specificity, and B.pahangi, B.malayi and W.bancrofti must share most of the common antigenic molecules. All antisera reacted well with the surface of L3 B.pahangi in the whole mount preparation. This indicates non-stage specificity. The most intense fluorescence was located at the epicuticle, the basal lamina lining the body wall, the gut and the reproductive tract, the egg shell in utero and the sperm. The hypodermis, the muscle cells, the cuticle beneath the epicuticle, the epithelial cells of the gut and the reproductive tract showed moderate fluorescence. The least fluorescence was observed in the egg interior.

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Published

01-06-2003

How to Cite

Roongruangchai, J. ., Pilakasiri, K. ., Roongruangchai, K. ., Pilakasiri, C. ., & Sobhon, P. . (2003). The Possible Antigenic Sources of Brugia pahangi. Siriraj Medical Journal, 54(6), 294–306. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245410

Issue

Vol. 54 No. 6 (2002): June

Section

Original Article

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