Modified Boosted - p24 Antigen can Predict HIV-1 RNA Viral Load (Ampiclor HIV-1 Monitor)

Authors

  • Nirun Vanprapar Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Ruengpung Sutthent Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Kulkanya Chokephaibulkit Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Wanatpreeya Phongsamart Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Pimpanada Chearskul Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Boosted-p24 antigen, HIV-1, Viral load, Prediction Likelihood ratio positive

Abstract

Objective: To evaluate the predictive value of HIV-1 RNA viral load (Ampiclor) in log-copies per ml by each level of modified boosted-p24 antigen in log-fg per ml.
Methods: 283 plasma samples were collected and blindly determined HIV-1 RNA Amplicor Monitor, Roche as a standard test with modified boosted p24 antigen assay. Likelihood ratio positive analyses of multiple levels of p24 were calculated as well as the post-test probability in predicting the amount of virus (log-copies/ml)
Results: Subjects were between 18 to 73 years old with the range of virus 1.75 to 5.92 log-copies per ml. By the calculation of likelihood ratio positive and positive predictive value, it was demonstrated that modified boosted p24 antigen (Ag in log-fg per ml) might predict the viral load (VL) in log-copies per ml as follow:

     Ag 2.0-3.0 log-fg per ml corresponding to 2.0 or lower log-copies per ml VL
     Ag 3.0-3.5 log-fg per ml corresponding to 2.5 or lower log-copies per ml VL
     Ag 3.5-4.0 log-fg per ml corresponding to 3.5 or lower log-copies per ml VL
     Ag 4.0-overlog-fg per ml corresponding to 4.5 or lower log-copies per ml VL

Conclusions: In countries where financial resources are limited, modified p24 antigen may be clinically applied in antiretroviral management program, instead of HIV-1 RNA Amplicor Monitor, Roche.

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Published

01-06-2005

How to Cite

Vanprapar, N. ., Sutthent, R. ., Chokephaibulkit, K. ., Phongsamart, W. ., & Chearskul, P. . (2005). Modified Boosted - p24 Antigen can Predict HIV-1 RNA Viral Load (Ampiclor HIV-1 Monitor). Siriraj Medical Journal, 57(6), 173–176. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/245593

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Original Article