Rapid Determination of Nevirapine in Human Plasma by High- Performance Liquid Chromatography

Authors

  • Kantima Sangsiriwut Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Thanomsak Anekthananon Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Winai Ratanasuwan Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Wichai Techasathit Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Surapol Suwanagool Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Surin Khunyok Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Teera Kolladarungkri Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

HPLC, HIV, nevirapine

Abstract

Objective: To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of nevirapine in human plasma.

Methods: A plasma sample and an internal standard were extracted with tert-butyl methyl ether and determined nevirapine concentration by HPLC method. The limit of quantitation (LOQ), accuracy, precision, specificity, stability and recovery were tested for method validation.

Results: Standard curve was linear in the range 0.1 μg/mL to 20 μg/mL. The limit of quantitation was 0.1 μg/ml. Coefficients of variation (CV) of intra-day and inter-day precision were less than 4%. Accuracy was range from 97-101%. The extraction recovery was range from 94-112%.

Conclusion: A rapid, sensitive and specific HPLC method was developed and can be used for determination of plasma nevirapine concentration in adult and pediatric patients infected with HIV.

Downloads

Published

01-01-2007

How to Cite

Sangsiriwut, K. ., Anekthananon, . T., Ratanasuwan, W. ., Techasathit, W. ., Suwanagool, S. ., Khunyok, S. ., & Kolladarungkri, T. . (2007). Rapid Determination of Nevirapine in Human Plasma by High- Performance Liquid Chromatography. Siriraj Medical Journal, 59(1), 8–11. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246022

Issue

Vol. 59 No. 1 (2007): Jan-Feb

Section

Original Article

License

Users are free to share, copy, and redistribute all articles published in the Siriraj Medical Journal (SMJ) in any medium or format as long as you follow the following terms:

  • Attribution — You must give appropriate credit, provide a link to the material, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the publisher endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • NoDerivatives — If you remix, transform, or build upon the material, you may not distribute the modified material.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.