Rapid Determination of Nevirapine in Human Plasma by High- Performance Liquid Chromatography

Authors

  • Kantima Sangsiriwut Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Thanomsak Anekthananon Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Winai Ratanasuwan Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Wichai Techasathit Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Surapol Suwanagool Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Surin Khunyok Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Teera Kolladarungkri Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

HPLC, HIV, nevirapine

Abstract

Objective: To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of nevirapine in human plasma.

Methods: A plasma sample and an internal standard were extracted with tert-butyl methyl ether and determined nevirapine concentration by HPLC method. The limit of quantitation (LOQ), accuracy, precision, specificity, stability and recovery were tested for method validation.

Results: Standard curve was linear in the range 0.1 μg/mL to 20 μg/mL. The limit of quantitation was 0.1 μg/ml. Coefficients of variation (CV) of intra-day and inter-day precision were less than 4%. Accuracy was range from 97-101%. The extraction recovery was range from 94-112%.

Conclusion: A rapid, sensitive and specific HPLC method was developed and can be used for determination of plasma nevirapine concentration in adult and pediatric patients infected with HIV.

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Published

01-01-2007

How to Cite

Sangsiriwut, K. ., Anekthananon, . T., Ratanasuwan, W. ., Techasathit, W. ., Suwanagool, S. ., Khunyok, S. ., & Kolladarungkri, T. . (2007). Rapid Determination of Nevirapine in Human Plasma by High- Performance Liquid Chromatography. Siriraj Medical Journal, 59(1), 8–11. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246022

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Original Article