Effects of Cellular Uptake of Flavonoids against Peroxynitrite-mediated Cell Cytotoxicity

Authors

  • Uraiwan Panich Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Walapha Ananta Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Tassanee Onkoksoong Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Kannika Jaemsak Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Antioxidant, cytotoxicity, flavonoids, peroxynitrite, uptake

Abstract

Objective: Flavonoids-derived antioxidant properties have been proposed to be beneficial to various pathologies associated with reactive species including peroxynitrite. It is thus crucial to investigate cellular uptake of flavonoids which are responsible for biological effects in target cells. The objectives of this study were to evaluate the uptake of flavonoids in human colon cancer (HT-29) and chondrosarcoma (SW1353) cells by assessing intracellular phenolic contents and effects against peroxynitritemediated SW1353 cytotoxicity.

Methods: The radical scavenging activities of flavonoids (catechin, epicatechin or quercetin) were determined using Thin layer chromatography-1,1-diphenyl-2-picrylhydrazyl (TLC-DPPH) assay. HT-29 and SW1353 cells were treated with the flavonoids and removed after 18-h incubation before the assay. Flavonoid uptake in HT-29 and SW1353 cells was demonstrated by assessing phenolic contents in the cell lysates using the Folin-Ciocalteau method. Additionally, their effects in inhibiting peroxynitrite-mediated SW1353 cell cytotoxicity were studied by using 4 cytotoxicity models: crystal violet-staining, 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) reduction, cellular glutathione (GSH) assay and intracellular oxidant formation.

Results: DPPH assay showed that quercetin exhibited the greatest radical scavenging activity. Phenolic contents detected in both HT-29 and SW1353 cell lines pre-incubated with quercetin were also greater in HT-29 cell lysates. SW1353 cytotoxicity by peroxynitrite was also inhibited by pre-incubation with quercetin. Additionally, phenolic contents and cytoprotective effects were not found in cells pre-incubated with catechin or epicatechin.

Conclusion: There appears to be an uptake of quercetin but neither catechin nor epicatechin in HT-29 and SW1353 cells. Moreover, accumulation of quercetin, the powerful free radical scavenger, in SW1353 cells may contribute to its cytoprotective effect against peroxynitrite-induced cytotoxicity.

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Published

01-09-2007

How to Cite

Panich, U. ., Ananta, W. ., Onkoksoong, T., & Jaemsak, K. (2007). Effects of Cellular Uptake of Flavonoids against Peroxynitrite-mediated Cell Cytotoxicity. Siriraj Medical Journal, 59(5), 237–241. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246139

Issue

Vol. 59 No. 5 (2007): Sep-Oct

Section

Original Article

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