Effect of Oxidative Stress on Leukocyte DNA Damage in β-thalassemic Patients

Authors

  • Sarun Kunwittaya National Institute for Child and Family Development, Mahidol University
  • Prasit Chanarat Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University
  • Yuttana Mundee Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University
  • Umnat Mevatee Department of Anatomy, Faculty of Medicine, Chiang Mai University

Keywords:

Comet assay, DNA damage

Abstract

Objective: Thalassemia is a group of genetic diseases where hemoglobin synthesis is impaired. Chronic anemia leads to increased dietary iron absorption, which develops into iron overload pathology. Treatment through regular transfusions increases oxygen capacity but also provides excess iron deposited in tissues. Thalassemia patients are particularly at risk of free radical induced damage. This study has investigated that effect of oxidative stress on leukocyte DNA damage in β-thalassemic patients.

Methods: Patients with post-splenectomized and transfusion dependent β-thalassemia major; age 4-15 years were recruited. Leukocyte DNA strand breakage was performed by single cell gel electrophoresis or comet assay which was analyzed as comet tail moment.

Results: Î²-thalassemic patients (n=30); age 4-15 years, 25 FA and 5 EF of hemoglobin typing. Peripheral blood mononuclear cell of β-thalassemic patients showed significant higher comet tail moment than normal control (165.17 ± 2.10 vs 28.0 ± 2.50 pixels, p < 0.05).

Conclusion: Peripheral blood mononuclear cell of β-thalassemic patients had an increased DNA damage when compared with normal control. This evidence demonstrated that free radical and iron overload might be a cause of leukocyte DNA damage.

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Published

01-09-2007

How to Cite

Kunwittaya, S. ., Chanarat, P. ., Mundee, Y., & Mevatee, U. . (2007). Effect of Oxidative Stress on Leukocyte DNA Damage in β-thalassemic Patients. Siriraj Medical Journal, 59(5), 242–244. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246140

Issue

Vol. 59 No. 5 (2007): Sep-Oct

Section

Original Article

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