Freezing Effect on Post-Thawed Sperm Characteristics Especially Sperm DNA Integrity Comparing between Liquid Nitrogen Vapour and Computerized Program Freezer

Authors

  • Somsin Petyim Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Roungsin Choavaratana Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Somboon Kunathikom Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Pitak Laokirkkiat Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Japarath Prechapanich Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Cryopreservation, computerized program freezer, liquid nitrogen vapour, sperm characteristics, sperm DNA integrity

Abstract

The aim of the study was to evaluate the cryodamage effects on human sperm characteristics, especially on sperm DNA integrity, after 6 months of freezing comparing between using liquid nitrogen vapour (LNV) and using computerized program freezer (CPF). Forty normal semen samples were collected for semen analysis. Each sample was mixed with cryoprotective media and divided into 2 straws. The first straw was frozen with LNV and the second one with CPF. After 6 months of cryostorage, semen samples were thawed, and sperm chromatin integrity as well as sperm motility, morphology, vitality and cryosurvival rate were determined. Percentages of DNA damage were higher (p<0.01) following freezing with LNV than with CPF. Sperm vitality was greater (p<0.05) after CPF than after LNV, as well as cryosurvival rate (p<0.001). Post-thawed sperm motility was greater after CPF than after LNV, either in grade A (p<0.001) or in grade B (p<0.05). No significant difference was observed in the percentage of normal sperm morphology comparing the two freezing methods. The current study demonstrated a post-thawed decrease in sperm DNA integrity as well as other sperm characteristics after freezing in both methods. The CPF significantly provided superior results in post-thawed sperm DNA integrity, sperm motility and vitality than LNV did. In case of 6 months of cryostorage, therefore, we recommend the computerized program freezer as a preference for sperm cryopreservation.

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Published

01-11-2007

How to Cite

Petyim, S. ., Choavaratana, R. ., Kunathikom, S. ., Laokirkkiat, P. ., & Prechapanich, J. (2007). Freezing Effect on Post-Thawed Sperm Characteristics Especially Sperm DNA Integrity Comparing between Liquid Nitrogen Vapour and Computerized Program Freezer. Siriraj Medical Journal, 59(6), 298–302. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246163

Issue

Vol. 59 No. 6 (2007): Nov-Dec

Section

Original Article

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