Detection of GSTM1 Polymorphism in Patient with Nasopharyngeal Carcinoma by Real-Time PCR

Authors

  • Danai Tiwawech National Cancer Institute
  • Somjin Chindavijak National Cancer Institute
  • Anant Karalak National Cancer Institute
  • Takafumi Ishida it of Human Biology and Genetics, Department of Biological Sciences, School of Science, University of Tokyo
  • Yaninee Jarratwisrutpom National Cancer Institute

Keywords:

Glutathione S-transferase M1 gene, melting curve, nasopharyngeal carcinoma, real-time polymerase chain reaction, SYBR green I

Abstract

Objective: To investigate whether the real-time polymerase chain reaction (R-PCR) assay with SYBR green I and melting curve analysis could be used for glutathione S-transferase M1 gene (GSTM1) polymorphism detection in Thai nasopharyngeal carcinoma (NPC) patients by comparing the results of this assay with the conventional PCR (C-PCR) assay.
Methods: DNA samples from peripheral blood leukocytes of 60 Thai NPC patients were investigated in this study. GSTM1 polymorphism [GSTM1 normal genotype (GSTM1+) and GSTM1 null genotype (GSTM1-)] were examined by using the R-PCR assay with SYBR green I and melting curve analysis and the C-PCR assay.
Results: The results of GSTM1 polymorphism detection by the R-PCR assay were in concordance with the C-PCR assay (κ= 1.0). Twenty-six individuals with GSTM1+ in the R-PCR assay showed 2 peaks of melting point at 82.5o C and 87.5o C that correlated with the appearance of 2 DNA bands of GSTM1 [215 base pair (bp)] and β-globin (268 bp) in the C-PCR assay, respectively. In addition, thirty-four individuals with GSTM1- in the R-PCR assay showed only 1 peak of melting point at 87.5o C that correlated with the appearance of 1 DNA band of β-globin (268 bp) in the C-PCR assay. Moreover, we found that the R-PCR assay was a faster and safer method for detection of GSTM1 polymorphism than the C-PCR assay.
Conclusion: The present study suggests that the R-PCR assay with SYBR Green I and melting curve analysis may be a useful screening tool for more convenient, rapid, reliable, and safer detection of GSTM1 polymorphism in Thai NPC as compared to the C-PCR assay.

Downloads

Published

01-01-2008

How to Cite

Tiwawech, D. ., Chindavijak, S. ., Karalak, A. ., Ishida, T., & Jarratwisrutpom, Y. (2008). Detection of GSTM1 Polymorphism in Patient with Nasopharyngeal Carcinoma by Real-Time PCR. Siriraj Medical Journal, 60(1), 23–27. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246304

Issue

Vol. 60 No. 1 (2008): Jan-Feb

Section

Original Article

License

Users are free to share, copy, and redistribute all articles published in the Siriraj Medical Journal (SMJ) in any medium or format as long as you follow the following terms:

  • Attribution — You must give appropriate credit, provide a link to the material, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the publisher endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • NoDerivatives — If you remix, transform, or build upon the material, you may not distribute the modified material.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.