Antiproliferative Effect of Cucurbitacin B Extracted from Trichosanthes cucumerina L. on Human Cancer Cell Lines

Authors

  • Tanawan Kummalue Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Weena Jiratchariyakul Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University
  • Totsaporn Srisapoomi Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Sathien Sukpanichnant Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Toshiro Hara Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University
  • Kenzaburo Tani Department of Molecular Genetics, Medical Institute of Bioregulation, Kyushu University

Keywords:

Trichosanthes cucumerina, Buap-Khom, cucurbitacin B, human cancer cell lines

Abstract

Objective: To determine the antiproliferative effect of cucurbitacin B extracted from Trichosanthes cucumerina L. on human
cancer cell lines.
Methods: Two human lung non-small cell (adenocarcinoma) cancer cell lines i.e., LK87, and QG95, two human colon
adenocarcinoma cell lines i.e., HCT15, and HT29, including one renal cancer cell line, A498, and one pancreatic cancer cell
line, NOR-P, were used in this study. The viability of cells was assessed by using WST-8 which is based on detection of
LDH released from damaged cells and reacts with WST-8 to form a yellow color. Cells were treated with the compound at
various concentration from 1 through 100 μg/ml.
Results: The ED50 values (effective doses that are required for 50% inhibition growth of tumor cells) of the compound on
human cancer cell lines ranged from approximately 69 μg/ml in HCT15 cells up to 231 μg/ml in QG95 cells. The inhibition
of proliferation of this compound on these human cancer cell lines was observed to be in a dose dependent manner.
Conclusion: It could be concluded from this observation that this compound has a modest direct toxic effect to these cell
lines with the highest toxic effect on human colon cancer cells.

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Published

01-01-2009

How to Cite

Kummalue, T. ., Jiratchariyakul, W. ., Srisapoomi, T. ., Sukpanichnant, S. ., Hara, T. ., & Tani, K. . (2009). Antiproliferative Effect of Cucurbitacin B Extracted from Trichosanthes cucumerina L. on Human Cancer Cell Lines. Siriraj Medical Journal, 61(2), 75–77. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246538

Issue

Vol. 61 No. 2 (2009): Mar-Apr

Section

Original Article

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