Optimal Level of Sections for Definite Diagnosis in Transbronchial Biopsy and Pleural Biopsy Specimens

Authors

  • Panthep Suttinont Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University
  • Usanee Nakphong Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Optimal level of section, transbronchial biopsy, pleural biopsy

Abstract

Objective: To determine optimal level of serial section in transbronchial and pleural biopsy that yield maximal definite
diagnosis.
Methods: A cross sectional study of 118 transbronchial biopsy and pleural biopsy specimens submitted with serial
sectioning in 3 levels were performed. Specimens of 1 mm. in diameter were serially cut and slides at levels I, II, III (120,
240 and 360 μm.) from initial exposure of tissue in paraffin blocks were studied, and specimens of 2-3 mm. in diameter
were cut at levels I, II, III (0, 120 and 240 μm.) after tissues in paraffin blocks were trimmed to expose maximal diameter.
Comparisons of diagnoses of each level were done.
Results: The percentages of definite diagnoses were 90.2, 96.1 and 93.2 in sections of level I, II and III, respectively.
Chronic granulomatous lesions were found in section level II more than other levels, but there was no statistical
significance. (P value 0.131, Chi-Square test)
Conclusion: Transbronchial and pleural biopsy specimens should be cut deep to level II, one slide for hematoxylin-eosin
staining and 3 unstained slides for further investigation.

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Published

01-05-2009

How to Cite

Suttinont, P. ., & Nakphong, U. . (2009). Optimal Level of Sections for Definite Diagnosis in Transbronchial Biopsy and Pleural Biopsy Specimens. Siriraj Medical Journal, 61(3), 123–125. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/246592

Issue

Vol. 61 No. 3 (2009): May-Jun

Section

Original Article

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