The Upregulation of Anti-apoptotic BIRC3 Expression Facilitates Zika Virus Infection

Anup Pandeya; Pucharee Songprakhon; Punnada Ruangsawat; Shilu Malakar; Sansanee Noisakran; Atthapan Morchang; Mongkol  Uiprasertkul; Chayawat Phatihattakorn; Prasert Auewarakul; Pa-thai Yenchitsomanus; Thawornchai  Limjindaporn

doi:10.33192/smj.v78i4.280320

Authors

Anup Pandeya Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Pucharee Songprakhon Division of Molecular Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Punnada Ruangsawat Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Shilu Malakar Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Sansanee Noisakran National Centre for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok, Thailand
Atthapan Morchang Cancer and Immunology Research Unit (CIRU), School of Medicine, Mae Fah Luang University, Chiang Rai, Thailand
Mongkol Uiprasertkul Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Chayawat Phatihattakorn Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Prasert Auewarakul Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Pa-thai Yenchitsomanus Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Thawornchai Limjindaporn Department of Anatomy, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

DOI:

https://doi.org/10.33192/smj.v78i4.280320

Keywords:

Apoptosis, BIRC3 gene, Zika virus infection

Abstract

Objective: The aim of this study was to investigate the apoptotic gene expression profile and to identify the genes involved in apoptosis and ZIKV infection.

Materials and Methods: SH-SY5Y cell line was infected with ZIKV at an MOI of 5. The cells were stained with Annexin V and propidium iodide to determine apoptosis. A real-time polymerase chain reaction array was employed to determine the apoptotic gene expression profile in a ZIKV-infected SH-SY5Y cell line. Western blot analysis was performed to confirm the expression of BIRC3 in both SH-SY5Y and A549 cell lines. Knockdown of the BIRC3 was done in ZIKV-infected A549 cell line using BIRC3 -specific siRNA. The ZIKV production was measured by focus-forming unit assay.

Results: Apoptotic genes in both extrinsic and intrinsic pathways, such as TNF-α, TRAIL, FAS, CASP8, CASP9, and BIRC3, were found to be upregulated. The anti-apoptotic gene BIRC3 was selected and found to be upregulated at the protein level in both ZIKV-infected SH-SY5Y and ZIKV-infected A549 cell lines. Knockdown of the BIRC3 gene in ZIKV-infected A549 cell line decreased Zika virus NS1 protein expression and Zika virion production.

Conclusion: The upregulation of anti-apoptotic BIRC3 expression facilitates Zika virus infection.

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