The Effect of Sample Storage Temperature on the Phenytoin Level in Serum

Authors

  • Paiboon Tummarintra Siriraj Poison Control Center, Siriraj Hospital, Faculty of Medicine Siriraj Hospital, Mahidol University Bangkok 10700
  • Phakrop Kaewbundit Siriraj Poison Control Center, Siriraj Hospital
  • Rasda Boonprasert Siriraj Poison Control Center, Siriraj Hospital
  • Teera Kolladarungkri Department of Preventive and Social Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University

Keywords:

Phenytoin, stability, storage

Abstract

Objective: To study the effect of temperature on phenytoin concentration in serum and to determine the sample were kept at 2-8°C and 35°C.
Methods: Serum samples of patients, who had been treated with phenytoin. had their phenytoin level determined on the first day. The sample was stored for seven days and analyzed on days three, five and seven of the study and divided into three storage concentrations from 2.5-10, 10.1-20 and 20.1-30 µg/ml compared using 50 samples per group.
Results: Phenytoin level was found to have increased over time to three days at a temperature of 35°C and five days at 2-8°C. It increased in the level of statistical significance at p<0.05. This study’s data were all well correlated at r2 = 0.9981-0.9999.
Discussion: Collected samples for analysis of phenytoin level must be stored at 2-8°C and stored for at least five days, to prevent  degradation of protein which cannot bind to the drug. The phenytoin levels in serum increased to make the diagnosis of dosage mistakes.

Keywords: Phenytoin, stability, storage

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Published

21-12-2015

How to Cite

Tummarintra, P., Kaewbundit, P., Boonprasert, R., & Kolladarungkri, T. (2015). The Effect of Sample Storage Temperature on the Phenytoin Level in Serum. Siriraj Medical Journal, 67(6), 262–266. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/55210

Issue

Vol. 67 No. 6 (2015): November-December

Section

Original Article

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Copyright (c) 2015 Siriraj Medical Journal

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