Bacopamonnieri Protects SH-SY5Y Cells Against tert-Butyl Hydroperoxide-induced Cell Death via the ERK and PI3K Pathways

Authors

  • Sukkid Yasothornsrikul Department of Biochemistry, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000

Abstract

Objective: Oxidative stress plays an important role in the pathological processes of various neurodegenerative diseases. Bacopa monnieri (BM) has a potent antioxidant property. Therefore, the purpose of this study was to evaluate the neuroprotective potential of BM against SH-SY5Y neuroblastoma cell death induced by the pro-oxidant insult, tert-Butyl hydroperoxide (TBHP), and to identify possible mechanisms related to its neuroprotective action.
Methods: The neuroprotective effect of BM was evaluated by the degree of protection against TBHP-induced cell death in human SH-SY5Y cells that was measured by calcein-AM assay. ERK1/2 and Akt phosphorylation was evaluated by immunoblotting.
Results: We found that BM exhibited protection against TBHP-mediated cytotoxicity. The neuroprotective effect of BM was abolished in the presence of either ERK1/2 or PI3K inhibitors. In addition, western blotting with anti-phospho-ERK1/2 and anti-phospho-Akt antibodies showed that BM increased both ERK1/2 and Akt phosphorylation.
Conclusion: These results suggest that BM by activation of ERK/MAPK and PI3K/Akt signaling pathways protects SH-SY5Y cells from TBHP-induced cell death.

Keywords: Bacopa monnieri, tert-Butyl Hydroperoxide, SH-SY5Y cells, ERK1/2, PI3K

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Published

24-02-2015

How to Cite

Yasothornsrikul, S. (2015). Bacopamonnieri Protects SH-SY5Y Cells Against tert-Butyl Hydroperoxide-induced Cell Death via the ERK and PI3K Pathways. Siriraj Medical Journal, 67(1). Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/55257

Issue

Vol. 67 No. 1 (2015): January-February

Section

Original Article

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