Effect of Abstinence Duration on Sperm Sex Ratio
Abstract
Objective: To evaluate the effect of abstinence duration and semen processing on sperm sex ratio and semen parameters.
Methods: 100 normozoospermic samples from men who had abstinence for 1 - 10 days were processed with combined Percoll gradients with swim-up method. The ratio of X- to Y-bearing spermatozoa was determined using fluorescence in situ hybridization. The ratio of X- to Y-bearing spermatozoa and semen parameters in fresh and prepared samples in correlation with abstinence duration were assessed.
Results: No statistically significant influence of abstinence duration on the mean ratio of X- to Y-bearing sper- matozoa was detected in fresh or prepared samples. The mean values of total sperm count and total motile sperm in fresh samples were significantly increased after abstinence of more than 3 days. In the prepared samples, the peaks of total sperm count and total motile sperm were noted in day 4 of abstinence while the percentage of morphologically normal spermatozoa remained constant during 1-10 days of abstinence.
Conclusion: There was no statistically significant effect of abstinence duration on the sperm sex ratio in either fresh or prepared samples. The total motile sperm in fresh samples were significantly increased after 4 days of abstinence. After sperm processing, the peak of the total motile sperm was observed at day 4 of abstinence while the abstinence duration did not affect the percentage of morphologically normal spermatozoa.
Keywords: Abstinence duration, sperm sex ratio, Percoll gradients, swim-up
Siriraj Med J 2014;66:103-112
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