The Effects of Ayurved Siriraj Wattana Recipe on Splenocytes in Wistar Rat
Keywords:
Wattana Ayurved Siriraj, splenocyte, immunostimulationAbstract
Wattana Ayurved Siriraj herbal formulary® has been used for decades and is quoted to carry anti-ageing action. Here, we have investigated the immunological efficacy and the safety for the consumption of Ayurved Siriraj Watana Recipe in Wistar rats. Rats were orally administered with Ayurved Siriraj Watana Recipe at a dose range of 0, 100, 300 and 1,000 mg/kg Body Weight (BW) for 14 days. After the treatments, splenocytes were isolated from these rats and examined for cell proliferation and NK cell activities. We found that the ingestion of Ayurved Siriraj Watana Recipe was not toxic to Wistar rats in all doses based on general gross and microscopic examinations. The recipe could not drive the proliferation of rat splenocytes in a dose-dependent manner. The ingested Ayurved Siriraj Watana Recipe also could not drive the NK cell cytotoxicity. Both the proliferative and NK cell assay revealed unfavorable response from rats ingesting 300 mg/kg BW AVS073. In summary, the ingestion of Ayurved Siriraj Watana Recipe in Wistar rats was safe with the studied dose. The use of Wistar rats for immunological screening of Ayurved Siriraj Watana Recipe or others is not suitable, since conventional mitogens also failed to stimulate their splenocytes. Other established animal models (e.g., BALB/c mice) would be more relevant.Downloads
Published
04-04-2017
How to Cite
Duangsa-ard, S., Wongkajornsilp, A., Akarasereenont, P., Huabprasert, S., Chongputtharaksa, T., & Laohapand, T. (2017). The Effects of Ayurved Siriraj Wattana Recipe on Splenocytes in Wistar Rat. Siriraj Medical Journal, 65(3), 73–76. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/82150
Issue
Section
Original Article
License
Users are free to share, copy, and redistribute all articles published in the Siriraj Medical Journal (SMJ) in any medium or format as long as you follow the following terms:
- Attribution — You must give appropriate credit, provide a link to the material, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the publisher endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- NoDerivatives — If you remix, transform, or build upon the material, you may not distribute the modified material.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.