The Effects of Ayurved Siriraj Wattana Recipe on Splenocytes in Wistar Rat

Abstract

Wattana Ayurved Siriraj herbal formulary® has been used for decades and is quoted to carry anti-ageing action. Here, we have investigated the immunological efficacy and the safety for the consumption of Ayurved Siriraj Watana Recipe in Wistar rats. Rats were orally administered with Ayurved Siriraj Watana Recipe at a dose range of 0, 100, 300 and 1,000 mg/kg Body Weight (BW) for 14 days. After the treatments, splenocytes were isolated from these rats and examined for cell proliferation and NK cell activities. We found that the ingestion of Ayurved Siriraj Watana Recipe was not toxic to Wistar rats in all doses based on general gross and microscopic examinations. The recipe could not drive the proliferation of rat splenocytes in a dose-dependent manner. The ingested Ayurved Siriraj Watana Recipe also could not drive the NK cell cytotoxicity. Both the proliferative and NK cell assay revealed unfavorable response from rats ingesting 300 mg/kg BW AVS073. In summary, the ingestion of Ayurved Siriraj Watana Recipe in Wistar rats was safe with the studied dose. The use of Wistar rats for immunological screening of Ayurved Siriraj Watana Recipe or others is not suitable, since conventional mitogens also failed to stimulate their splenocytes. Other established animal models (e.g., BALB/c mice) would be more relevant.