Determination of KISS1, KISS1R and Kisspeptin in Fat Tissue of Normal Weight and Obese Humans and Correlations between Serum Kisspeptin and Leptin

Authors

  • Chantacha Sitticharoon
  • Vipada Boonpuan
  • Chalermchai Mitrpant
  • Malika Churintaraphan

Keywords:

KISS1, KISS1R, kisspeptin, leptin, fat tissue

Abstract

Kisspeptin, a neuropeptide mainly expressed in the hypothalamus, has a major role in reproductive regulation. Previous studies showed that Kiss1 mRNA expression is also found in adipose tissue in rats. This study aimed to determine KISS1, KISS1R, and leptin expression in fat tissues. Both KISS1 and KISS1R mRNA expressions were extremely low in human subcutaneous and visceral adipose tissues in real-time PCR analysis. Nested real-time PCR was done for both KISS1 and KISS1R primers showing that the expressions of KISS1 and KISS1R mRNA were highly determined in visceral, but not in subcutaneous adipose tissues. Serum kisspeptin levels were not different between obese and normal weight subjects and were positively correlated with serum leptin (R=0.381, p<0.05). In conclusion, KISS1 and KISS1R expressions were detected in visceral adipose tissue.

 

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Published

06-04-2017

How to Cite

Sitticharoon, C., Boonpuan, V., Mitrpant, C., & Churintaraphan, M. (2017). Determination of KISS1, KISS1R and Kisspeptin in Fat Tissue of Normal Weight and Obese Humans and Correlations between Serum Kisspeptin and Leptin. Siriraj Medical Journal, 65(4), 112–116. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/82333

Issue

Vol. 65 No. 4 (2013): July-August

Section

Original Article

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