Antioxidative and Neuroprotective Activities of Oroxylum indicum Stem Bark Extracts in Mouse Neuroblastoma Cells
Keywords:
Oroxylum indicum, antioxidative activity, neuroprotective activityAbstract
Oxidative stress is a major risk factor for neurodegenerative diseases. It has been reported that antioxidants could relieve symptoms of neurodegenerative diseases. Pa-ka (Oroxylum indicum (L.) Kurz) stem bark contains polyphenols with antioxidant activities and may be use to alleviate oxidative stress-induced neurodegenerative process. This study aimed to investigate the antioxidative and neuroprotective activities of water extract (OI-W) and ethanol extract (OI-E) from Pa-ka stem bark on hydrogen peroxide and glutamate-induced neurotoxicity in N1E-115 cell cultures. For free radical scavenging capacity using hydroxyl and superoxide scavenging assay, OI-W and OI-E exhibited antioxidant activity. It was found that co-exposure of cultured neurons to hydrogen peroxide with OI-W or OI-E extract for 24 hours could protect against cell injury or cell death from hydrogen peroxide in N1E-115 cultures, while both extracts did not prevent glutamate-induced neurotoxicity. These findings indicated that O. indicum stem bark showed the neuroprotective effect on hydrogen peroxide-induced neurotoxicity, at least, its mechanism might partly involve the antioxidant activity. Further studies in in vitro models with specific pathology as well as in animals are required to clarify its activity and underlying mechanisms prior to develop as herbal medicine for the treatment of neurodegenerative diseases.
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