Global DNA Methylated Gene of Involved Skin and Uninvolved Skin of Psoriasis Compared with Normal Skin by Methylation Microarray

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Kriangsak Ruchusatsawat
Laddawan Thiemsing
Protjaroen Phantipa
Jongkonnee Wongpiyabovorn

Abstract

       Psoriasis is approximately 1-3% of the population worldwide. Etiology of the disease is still unclear. Recently, epigenetic phenomena have also been implicated in the pathogenesis of psoriasis. We investigated DNA methylation by methylation microarray in tissue from 6 involved skins and uninvolved skins of chronic plaque type psoriasis patients compared to 6 normal skins from healthy controls. There are 6 candidate genes MSX1, TCP11, C16orf47, HOXB1, HTR3B and TH. MSX1 gene was selected from methylation microarray data and was analyzed DNA methylation correlated with genes expression in keratinocytes and peripheral blood mononuclear cells (PBMC) compared between psoriasis and normal controls, using methylation specifi c primer (MSP). DNA methylation occurred in three of four samples of normal skins, accounting for 75%. Whereas DNA methylation from 6 of all 6 involved skin samples were represented 0%. In conclusion, the present study is the fi rst study using high through-put technique (methylation microarray) to investigate DNA methylation in psoriasis. The result of methylation microarray could indicate the signifi cant the candidate genes for further studying in psoriasis. Moreover, this study demonstrated DNA methylation plays an important role in pathogenesis and course of psoriasis

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