The Cannabinoid Contents in Different Growth Stages of Thai Cannabis sativa L. Leaves
Keywords:
Cannabis sativa, Cultivar, CannabinoidsAbstract
Cannabinoids are the major compounds in cannabis containing tetrahydrocannabinol (THC), which is a psychoactive compound and other non-psychoactive compounds, such as cannabinol (CBN), cannabidiol (CBD). Cannabinoids are mainly found in inflorescence of cannabis, but sut some of them are also detected in other parts including leaves. The information of cannabinoids contents in cannabis leaves is still limited, especially in Thai cannabis. This study, therefore, aims to investigate cannabinoids contents using the Ultra High Performance Liquid Chromatography (UHPLC) technique in leaves of different growth stages. Four cultivars of Thai cannabis including Hang Kra Rog Phu Phan (ST1), Hang Suea Sakonnakhon (TT1), Tanao Si Kan Khaw (WA1) and Tanao Si Kan Dang (RD1) were grown and recorded leaf sizes as well as THC and CBD contents in different growth stages. The results showed that the immature leaves (both fresh and dried) of 2-month-grown ST1 and RD1 gave the highest weight compared to others. The immature and mature leaf stalks of RD1 were higher in length than others, but not different in width and length of their leaves. The highest THC content was detected in 2- month young leaves cannabis, while CBD content showed the highest (3.83 %w/w) in 1-month young leaves of RD1. In contrast, a small amount of CBN was detected in all cannabis
leaves. The information reported here could appropriately support medical use and create value-added for Thai cannabis.
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