Preparation of Blood Alcohol Proficiency Testing Sample in Thailand
Keywords:
Proficiency testing samples, Blood alcohol, Homogeneity, StabilityAbstract
Blood alcohol proficiency testing (PT) samples as prepared from bovine blood are designed to use as a tool for quality assessment of blood alcohol testing laboratory. PT samples must be similar to the testing samples and homogeneity as well as stability should be evaluated before distributing to participants. The objective of this study was to develop a new PT sample preparation technique to overcome the stability problem. The blood alcohol level was determined using Headspace Gas Chromatography-Flame Ionization Detector. According to ISO 13528:2015, homogeneity and stability results showed that the blood sample should be from the same cow and sodium fluoride (as preservative), and potassium oxalate (as anti-clotting agent) at the concentrations of 5 and 4 mg/ml, respectively, should be added. The mixtures should be mixed for 12 hours at 20 ± 2 ๐C and kept at 2-8 ๐C for 7 days. The PT samples were then prepared by adding different contents of alcohol to obtain appropriate concentrations covering the legal limit of 50 mg%.
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