Molecular laboratory testing for dengue serotypes at Bamrasnaradura Infectious Diseases Institute

Authors

  • Sumonmal Uttayamakul Bamrasnaradura Infectious Diseases Institute, Department of Disease Control
  • Ravee Nitiyanontakij Bamrasnaradura Infectious Diseases Institute, Department of Disease Control
  • Supatsa Dedsatit Bamrasnaradura Infectious Diseases Institute, Department of Disease Control
  • Sarinee Reawrang Bamrasnaradura Infectious Diseases Institute, Department of Disease Control
  • Patama Suttha Bamrasnaradura Infectious Diseases Institute, Department of Disease Control
  • Visal Moolasart Bamrasnaradura Infectious Diseases Institute, Department of Disease Control

Keywords:

dengue, serotype, molecular testing

Abstract

       Dengue virus (DENV) infection is a major serious health problem in many tropical countries including Thailand. DENV comprises of 4 serotypes (DENV-1 to DENV-4) and co-circulation of DENV and Chikungunya virus (CHIKV) has been reported in areas where dengue is common. This study aims to investigate genetic materials of DENV and CHIKV including serotype distribution of DENV in dengue-suspected patients using real-time polymerase chain reaction (real-time PCR). The study was conducted during 2016-2017 at Bamrasnaradura Infectious Diseases Institute and a total of 100 patients (29 children and 71 adults) were enrolled. The laboratory findings in dengue-suspected patients was screened by platelet count of ≤ 100,000 cells/μl, DENV NS1 antigen positive, or anti-DENV IgM positive. From 100 DENV-suspected cases, 37 cases were detected for DENV by real-time PCR. No CHIKV was detected. The results showed that 27 cases (73%) were admitted to the hospital, 18 cases (48.6%) had platelet count <100,000 cells/μl, 37 cases (100%) had NS1 antigen positive and 17 cases (45.9%) had anti-DENV IgM positive. The distribution of DENV-2, DENV-4, DENV-3 and DENV-1 serotypes was 43.2%, 32.4%, 18.9% and 5.4%, respectively. Rapid screening test results for NS1 antigen were concordant with those by real-time PCR. Serotype distribution using molecular testing could be useful for
studies on correlation between viral diversity and host immune response.

References

Gibbons RV, Vaughn DW. Dengue: an escalating problem. BMJ. 2002; 324: 1563–6.

Gubler DJ. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol. 2002; 10: 100–3.

Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature 2013; 496: 504-7.

World Health Organization. Dengue: guidelines for diagnosis, treatment, prevention and control. WHO/HTM/NTD/DEN/2009.1. Geneva: World Health Organization; 2009.

Kalayanarooj S, Vangveerawong M, Vatcharasaevee V, editorials. Guidelines for diagnosis and treatment of dengue hemorrhagic fever. Nonthaburi, Thailand: Ministry of Public Health; 2013.

Kauntner I, Robinson MJ, Kuhnle U. Dengue virus infection: epidemiology, pathogenesis, clinical presentation, diagnosis and prevention. J Pediatr 1997; 131: 516-24.

Kim Lien Pham Thi. Epidemiology and dynamic of dengue and chikungunya in several provinces in Vietnam. Microbiology and Parasitology. English: Université Montpellier; 2015.

Sessions OM, Wilm A, Kamaraj US, Choy MM, Chow A, Chong Y, et al. Analysis of dengue virus diversity during human and mosquito infection reveals genetic constraints. PLoS Negl Trop Dis 2015; 9(9): e0004044. (21 p.).

Holmes EC, Twiddy SS. The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol 2003; 3: 19-28.

Bureau of Epidemiology, Department of Disease Control, Ministry of Public Health. Annual epidemiological surveillance report. Nonthaburi, Thailand: Ministry of Public Health; 1977-1998.

Halstead SB, Yamarat C. Recent epidemics of hemorrhagic fever in Thailand, observations related to pathogenesis of a “new” dengue disease. Am J Public Health Nations Health 1965; 55: 1386-95.

Bureau of Epidermiology, Deparment of Disease Control, Ministry of Public Health. Annual epidemiological surveillance report 2003. Nonthaburi, Thailand: Ministry of Public Health; 2003.

Soo KM, Khalid B, Ching SM, Chee HY. Meta-analysis of dengue severity during infection by different dengue virus serotypes in primary and secondary infections. Plos One 2016; 11(5): e0154760. (16 p.).

Suwanmanee S, Surasombatpattana P, Soonthornworasiri N, Hamel R, Maneekan P, Missé D, et al. Monitoring arbovirus in Thailand: surveillance of dengue, chikungunya and zika virus, with a focus on coinfections. Acta Trop 2018; 188: 244-50.

Liulak W, Saichaer P, Waidab W, Pungjitprapai A, Buranakitsin S, Suntorn-opas B, et al. (2017). Prospective study of dengue in Bangkok during 2015-2016. Southeast Asian J Trop Med Public Health, 2017; 48: 33-38.

Yung CF, Lee KS, Thein TL, Tan LK, Gan VC, Wong JG, et al. Dengue serotype-specific differences in clinical manifestation, laboratory parameters and risk of severe disease in adults, Singapore. Am J Trop Med Hyg 2015; 92(5): 999-1005.

Fried JR, Gibbons RV, Kalayanarooj S, Thomas SJ, Srikiatkhachorn A, Yoon IK, et al. Serotype-specific diffenceces in the risk of dengue hemorrhagic fever: an analysis of data collected in Bangkok, Thailand from 1994 to 2006. PLoS Negl Trop Dis 2010; 4(3): e617. (6 p.).

Downloads

Published

28-01-2022

How to Cite

1.
Uttayamakul S, Nitiyanontakij R, Dedsatit S, Reawrang S, Suttha P, Moolasart V. Molecular laboratory testing for dengue serotypes at Bamrasnaradura Infectious Diseases Institute. ว กรมวิทย พ [internet]. 2022 Jan. 28 [cited 2025 Dec. 28];61(1):31-9. available from: https://he02.tci-thaijo.org/index.php/dmsc/article/view/240954

Issue

Vol. 61 No. 1 (2019): January - March 2019

Section

Laboratory Findings

License

Copyright (c) 2020 BULLETIN OF THE DEPARTMENT OF MEDICAL SCIENCES

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.