Method Development and validation for analysis of Dioxins, Dioxin-like PCBs and Non Dioxin-like PCBs in Milk Powder by HRGC/HRMS
Keywords:
Dioxins, Dioxin-like PCBs, indicator PCBs, milk powder, extraction, HRGC/HRMSAbstract
Broad toxic and biochemical effects for PCDDs/Fs, DL-PCBs, and NDL-PCBs have been reported in laboratory animals. The most sensitive endpoints are developmental neurobehavioral effects, developmental reproductive effects and immunotoxic effects. More than 90% of human exposure is through food, mainly meat, fish and shellfish, milk and dairy products. It is widely known that the determination of PCDDs/Fs and DL/NDL-PCBs requires special expertise, sophisticated instrumentation as well as a lot of time, manpower and chemical substances. The objective of this study is to develop and to validate a method of analysis of PCDDs/Fs and DL/NDL-PCBs in milk powder. Based on EPA 1613B and 1668B method, the method had been modified by increasing sample weight, changing and adjusting some chemical substances in extraction, cleaning-up procedures and separation of PCDDs/Fs, DL-PCBs and NDL-PCBs in which the limits of quantitation correspond to 0.06 – 0.64, 1.85 and 3.50 pg/g fat, respectively.
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