Quantitative Analysis of Imperatorin in Bael fruit by Ultra Performance Liquid Chromatography (UPLC)
Abstract
Imperatorin is an active principle for anti-oxidation, anti-tumor, vasodilation, anti-platelet aggregation in Bael fruit (Aegle marmelos (L.) Corrêa, Family Rutaceae). Although this plant is widely used in traditional medicine and health products, there is no quality control method for it. The objective of this study is to develop an analytical method for determination of imperatorin content in Bael fruit using UPLC. Sample preparation was prepared by reluxing 1 g of powdered drug in 50 ml of hexane for 30 min, iltering and evaporating until dryness. The residue was redissolved and adjusted with methanol to 25 ml. A 3 μl-portion of sample solution was injected to UPLC system. The analysis was performed using BEH C18, 2.1×50 mm, 1.7 μm column and 60% methanol as the mobile phase at low rate of 0.7 ml/min, and detected with PDA at wavelength 302 nm. This method was validated. Linearity was established for imperatorin concentration range of 0.0402-0.2414 mg/ml with a coeicient of determination (R2) 0.999896. %Recovery was in the range of 99.43-103.00%. HORRAT was in the range of 0.60-0.88. LOD and LOQ were equal to 0.0026 and 0.0086 mg/ml, respectively. It was shown that this developed method was suitable and could be used to analyze the content of imperatorin in Bael fruit. The appropriate speciication of imperatorin content in Beal fruit could be established.
References
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