Expansion of Cord Blood Mesenchymal Stem Cell Using Culture Medium Supplemented with Umbilical Cord Serum

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Suparat Wichitwiengrat
Sasiprapa Thongbopit
Suphakde Julavijitphong
Piengchia Vichaidith
Pussara Hakularb
Tatsanee Phermthai

Abstract

      Nowadays, the cultivation of cord blood mesenchymal stem cells (CBSC) can only propagated in a medium supplemented with embryonic stem cell grade fetal bovine serum (ES-FBS). By using a commercial human serum, the CBSC had been limited in cell propagation. Our previous study had showed a successful establishment of animal free-amniotic fluid stem cell (AFS), using human umbilical cord blood serum (hUCS). The current work is based on the idea of using hUCS for CBSC culture as a replacement of ES-FBS. With 49 cord blood samples for MSC isolation, every samples were cultured under the medium supplemented with hUCS comparable to supplementation with ES-FBS. The result demonstrated that CBSC lines generated under hUCS-medium showed similar morphology and specific MSC characteristics to the lines as from ES-FBS-medium. Both cell lines expressed CD29, CD73, CD90 and CD105 without expression of CD34 and CD45. Moreover, they can be differentiated into adipocyte and osteoblast. Nevertheless, the proliferation rate of CBSC derived in ES-FBS-medium was higher than the lines generated in hUCS-medium. The author concluded that hUCS can be used to replace ES-FBS in the medium for derivation and culture of CBSC lines.

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