Acute Oral Toxicity of Crocodylus siamensis Bile in Sprague Dawley Rats
Keywords:
Crocodile Bile, Acute Oral Toxicity Testing, Crocodylus siamensisAbstract
Crocodile bile is consisted of coprocholic acid, coprochenodeoxycholic acid, cholestanol, and other derivatives. In many countries, bile of animals has been used as an ingredient of traditional medicine. Although usage of Crocodylus siamensis (Siamese crocodile) bile has been used for treatment of various symptoms, toxicological studies of crocodile bile have not been evaluated. This study was to determine acute oral toxicity of C. siamensis bile in Sprague Dawley rats. Our study was conducted in a stepwise procedure and used fixed dose of C. siamensis bile at 300 and 2,000 mg/kg body weight according to OECD Guidelines for the testing of chemicals 420, Acute Oral Toxicity-Fixed Dose Procedure. The results showed that no mortality of rats orally given dried C. siamensis bile at the dose of 300 mg/kg body weight. In contrast, the dose of 2,000 mg/kg body weight demonstrated mortality within 24 hours after testing. The results suggested that C. siamensis bile is classified in Globally Harmonized System for Classification and Labeling of Chemicals as category 4 (300 mg/kg <LD50 ≤2,000 mg/kg).
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