Method Verification of Isolated Chicken Eye Test
Abstract
Alternative studies, using non-animal methods, for safety tests on healthcare products are increasing. Draize rabbit eye test is one of the methods that can be replaced by cell-based assays or organotypic assays. This study was to conduct and to verify Isolated Chicken Eye test (ICE) according to OECD Test Guideline 438. A total of 24 chemicals with classification as categorized by United Nations Globally Harmonized System of Classification and Labelling of Chemicals were used in the study. Our results demonstrated that specificity, sensitivity, accuracy, false positive rate and false negative rate were at percentages of 93, 78, 88, 7 and 22, respectively. Our data indicated that the method verified is acceptable to be used to replace the rabbit tests for identifying chemicals inducing serious eye damage in our animal laboratory.
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