Evaluation of Synthetic Antimicrobial Peptides against Methicillin-Resistant Staphylococcus aureus
Keywords:
Methicillin-resistant Stahylococcus aureus (MRSA), Peptide barcode, Synthetic peptidesAbstract
In this study, nine peptides containing 6-25 amino acids residues with positive charge and 40-83% hydrophobicity were selected from an antimicrobial peptide database. All of them were chemically synthesized and evaluated for their antibacterial activity against Staphylococcus aureus ATCC 25923, methicillin-resistant Staphylococcus aureus (MRSA) DMST 20635, MRSA DMST 20637 and MRSA DMST 20654. It was found that KLKLLLLLKLK had effective antibacterial activity against all S. aureus. This peptide was then modified and yielded twelve peptides with different hydrophobicity and charges. In the antibacterial assay with these twelve synthetic peptides, KLKLKLKLKLK, LLLLLLKLK and LLLLLLK were found to have potent antibacterial activity against S. aureus ATCC 25923, MRSA DMST 20635 and MRSA DMST 20654 with IC50 values of 27.5-62.5 μg/ml. Furthermore, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis results showed that peptide barcodes of all S. aureus strains were significant different. The results from this study indicated that the different degree of methicillin-resistant S. aureus has an effect on difference in peptide barcode and difference in response to synthetic peptides. However, the antibiotic mechanism of these three modified peptides against MRSA should be further studied for development as therapeutic agents.
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