Method Validation of Alcohol Contents in Alcohol-based Hand Sanitizer Products Using Gas-chromatography

Abstract

        Since the situation of COVID-19 infection around the world has still been widespread. Therefore, the alcohol-based sanitizer products are used popularly for washing hand without water. In Thailand, according to the Notification No. 137, Special Part 54, (2020) of the Ministry of Public Health, only ethyl alcohol, isopropyl alcohol or n-popyl alcohol are used in alcohol based hand sanitizer products which its total content shall not be less than 70% v/v. This study was aimed to develop and validate the method to determine three types of alcohol simultaneously using Gas-chromatography with Flame Ionization Detector (GC-FID). The test conditions were composed of DB-624 column with oven temperature program from 40-150 C. The method was specific. The linearity and working range were shown between 1.0-5.0 mg/mL with r >0.9999. The %recovery was 99.0-103.0%. The precision, within day, between days and analysts, were acceptable with RSD <3%. Limit of quantitation (LOQ) was 1.81, 1.76 and 1.80%v/v, respectively, while limit of detection (LOD) was 0.18 %v/v. Measurement uncertainty was equal to 0.05 %v/v. As the result, the developed method was suitable to be used for alcohol contents analysis in alcohol-based hand sanitizer products.