Development and Validation for Determination of Adventitious Viruses in Vero Cells Used for Vaccine Production by Using Indicator Cells
Keywords:
Vero cells, Adventitious viruses, Vaccine productionAbstract
A Vero cell substrate used to produce vaccines requires strict quality control. The determination of adventitious viruses by using indicator cells is one of the tests according to the WHO requirements. Therefore, the objectives of this study were to develop and validate a testing method to determine adventitious viruses in Vero cells. The optimal concentration of three indicator cells including Vero 76, MRC-5 and Vero cells (the same species used in the vaccine production) and the titers of two representative measles virus and bovine parainfl uenza virus type 3 (BPIV3) were analyzed. In this study, a Vero cell culture (1 × 107 cells/mL) was used for detection of viral contamination with co-culture in three indicator cells by observation of cytopathic eff ect (CPE), hemadsorption and hemagglutination. The results showed that this method had specifi city that was positive to measles virus and BPIV3 at the limitation of lowest titers of 0.1 and 0.01 CCID50/mL, respectively. It is thus indicated that this method could be benefi cial to determine the contamination of adventitious viruses in Vero cells used for vaccine production in laboratory.
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