SKIN NOURISHING SERUM PRODUCT DEVELOPMENT CONTAINING OF HELIANTHUS ANNUUS EXTRACT

Abstract

This research studied the antioxidant activity (DPPH assay and ABTS^+. assay) and total phenolic content (TPC) of sunflower sprout extracts. The skincare serum formulations were developed, and their stability was investigated under real time and accelerated (heating and cooling cycle) conditions. Nine-day-old sunflower sprouts were extracted using three solvents: 70% ethanol, 95% ethanol, and water. The antioxidant results (DPPH assay) showed that the water extract exhibited the highest activity, followed by the 95% ethanol and 70% ethanol extracts. The IC₅₀ values were 0.14–0.23 mg/ml, respectively. Results from the ABTS assay indicated that the water extracts had the best activity, followed by the 70% ethanol and 95% ethanol extracts. The IC₅₀ values ranged from 0.04 to 0.08 mg/ml. The total phenolic contents of the water, 70% ethanol, and 95% ethanol extracts were 25.42 ± 0.04, 17.55 ± 0.01, and 16.56 ± 0.22 mg/g gallic acid equivalent, respectively. Additionally, skincare serum formulations were developed from sunflower sprout extracts, using Carbopol 940 as a gelling agent. The serum showed consistency up to 180 days after accelerated stability testing and demonstrated suitable physical and chemical properties. Thus, sunflower sprout extract is suitable for use in skincare cosmetics.