Effect of Centotheca lappacea (L.) Desv Preparation Processes to Total Phenolic Compound and Antioxidant Activities

Abstract

Ya Repair (Centotheca lappacea (L.) Desv) has been used following Thai traditional medicine principles in order to help restore and nourish skin and body. Despite there being no studies on the effect of repair grass preparation before use to total phenolic compound and antioxidant activities (DPPH and ABTS assay). The objective of this research was to process the repair grass by four different methods, namely aeration, drying, flame grilling, and roasting, then determining total phenolic contents and antioxidant activities. The results showed that repair grass had total phenolic compounds of 22.07±8.10, 28.50±2.86, 92.12±5.33, and 121.39±2.57 µg gallic acid equivalent (GAE)/g dry weight. The antioxidant activities were reported by the half maximal inhibitory concentration (IC₅₀). TheIC50 of DPPH method were 170±0.02, 100±0.01, 80±0.01, and 60±0.01 mg/ml, and the ABTS method were 0.38±0.07, 0.24±0.02, 0.11±0.01, and 0.12±0.00 mg/ml from aeration, drying, flame grilling, and roasting method, respectively. The result showed that Ya repair with the roasting method had the highest phenolic compound and high antioxidant activities. Therefore, roasted repair grass could be a suitable method for future health supplement product development.