Development of Skin Care Lotion from Kaesorn-Tang-Ha Remedy Extract with In vitro Antioxidant and Anti-inflammatory activities

Abstract

Kaesorn-Tang-Ha remedy consists of medicinal plants to possess antioxidant property, which is able to prevent skin aging, resulting in maintaining skin hydration. A cosmeceutical product from Kaesorn-Tang-Ha remedy has not been reported. This research aimed to develop lotion from Kaesorn-Tang-Ha remedy extract with antioxidant and anti-inflammatory activities. The ethanol extract of Kaesorn-Tang-Ha remedy was assessed antioxidant activities using ABTS and DPPH assays and anti-inflammatory activity via inhibition of nitric oxide (NO) production using Griess method. Then the extract was used to formulate a lotion. The results showed that the extract of Kaesorn-Tang-Ha remedy had the high ABTS^•+ and DPPH^• radical scavenging activities with IC50 values of 0.09±0.01 and 0.05±0.01 mg/mL, respectively, and was able to inhibit NO production with IC50 value of 0.07±0.01 mg/mL. This extract was formulated lotions at concentrations of 0.01%, 0.10%. and 0.50% w/w. The accelerated stability of the herbal lotions was tested using heat-cool cycling method and the antioxidant and anti-inflammatory effectiveness of herbal lotions were evaluated. Resulting from this study, all formulations showed the good stability and no change in physical and chemical characteristics. Moreover, all herbal lotion formulations possessed antioxidant and anti-inflammatory potentials. In conclusion, lotions containing the extract of Kaesorn-Tang-Ha remedy at concentrations of 0.01%, 0.10%. and 0.50% w/w are worthy of further development as lotion/herbal product for antioxidation and anti-inflammation.