The Antioxidant Activity and Anti Staphylococcus aureus and Escherichia coli from Boesenbergia rotunda (L.) Mansf. Extract

Abstract

This study investigated the effect of inhibiting the growth of bacteria Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), as well as the antioxidant activity by DPPH radical scavenging assay method, total phenolic content and total flavonoid content of crude extracts from Boesenbergia rotunda (L.) Mansf. using two types of solvents with different polarities. The crude extracts of Boesenbergia rotunda (L.) Mansf. using methanol solvent produced the highest percentage yield when compared to dichloromethane solvent. The percentage yields of the crude extracts were 17.2% and 9.8%, respectively. The methanol extract of Boesenbergia rotunda (L.) Mansf. inhibited the growth of S. aureus with an average inhibition zone of 13.07±0.7841 mm, while E. coli showed no inhibition zones. The solvent dichloromethane did not exhibit any inhibition zones against either type of bacteria. Furthermore, crude extracts from Boesenbergia rotunda (L.) Mansf. using methanol had a total phenolic content of 1.220±0.072 mgGAE/gDW and total flavonoid content of 2.543±0.106 mgQE/gDW, while dichloromethane had 1.270±0.014 mgGAE/gDW and 3.512±0.027 mgQE/gDW, respectively.