The Antioxidant Activity and Anti Staphylococcus aureus and Escherichia coli from Boesenbergia rotunda (L.) Mansf. Extract
Main Article Content
Abstract
This study investigated the effect of inhibiting the growth of bacteria Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), as well as the antioxidant activity by DPPH radical scavenging assay method, total phenolic content and total flavonoid content of crude extracts from Boesenbergia rotunda (L.) Mansf. using two types of solvents with different polarities. The crude extracts of Boesenbergia rotunda (L.) Mansf. using methanol solvent produced the highest percentage yield when compared to dichloromethane solvent. The percentage yields of the crude extracts were 17.2% and 9.8%, respectively. The methanol extract of Boesenbergia rotunda (L.) Mansf. inhibited the growth of S. aureus with an average inhibition zone of 13.07±0.7841 mm, while E. coli showed no inhibition zones. The solvent dichloromethane did not exhibit any inhibition zones against either type of bacteria. Furthermore, crude extracts from Boesenbergia rotunda (L.) Mansf. using methanol had a total phenolic content of 1.220±0.072 mgGAE/gDW and total flavonoid content of 2.543±0.106 mgQE/gDW, while dichloromethane had 1.270±0.014 mgGAE/gDW and 3.512±0.027 mgQE/gDW, respectively.
Article Details
References
Gomaa EZ. (2017). Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria. The Journal of general and applied microbiology. 63(1), 36-43.
Kumar JD, et al. (2009). Detection of virulence genes in Staphylococcus aureus isolated from paper currency. International Journal of Infectious Diseases. 13(6), e450-e455.
Fisher JF & Mobashery S. (2020). β-Lactams against the Fortress of the Gram-Positive Staphylococcus aureus Bacterium. Chemical reviews. 121(6), 3412-3463.
Brown SP, et al. (2012). Evolution of virulence in opportunistic pathogens: generalism, plasticity, and control. Trends in microbiology. 20(7), 336-342.
Feazel LM, et al. (2009). Opportunistic pathogens enriched in showerhead biofilms. Proceedings of the national academy of sciences. 106(38), 16393-16399.
Bamberger DM & Boyd SE. (2005). Management of Staphylococcus aureus infections. American family physician. 72(12), 2474-2481.
Ryu S, et al. (2014). Colonization and infection of the skin by S. aureus: immune system evasion and the response to cationic antimicrobial peptides. International journal of molecular sciences. 15(5), 8753-8772.
Del Rio A, et al. (2009). Patients at risk of complications of Staphylococcus aureus bloodstream infection. Clinical Infectious Diseases. 48(Sup4), S246-S253.
He H & Wunderink RG. (2020). In Staphylococcus aureus pneumonia in the community, Seminars in respiratory and critical care medicine. Thieme Medical Publishers. pp 470-479.
Enright MC, et al. (2002). The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proceedings of the National Academy of Sciences. 99(11), 7687-7692.
Grema HA, et al. (2015). Methicillin resistant Staphylococcus aureus (MRSA). a review.
Köck R, et al. (2010). Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Eurosurveillance. 15(41).
Lim JY, et al. (2010). A brief overview of Escherichia coli O157: H7 and its plasmid O157. Journal of microbiology and biotechnology. 20(1), 5.
Slutsker L, et al. (1997). Escherichia coli O157: H7 diarrhea in the United States: clinical and epidemiologic features. Annals of internal medicine. 126(7), 505-513.
Rivas M, et al. (2000). Intestinal bleeding and occlusion associated with Shiga toxin producing Escherichia coli 0127: H21. MEDICINA-BUENOS AIRES. 60(2), 249-252.
Bell BP, et al. (1997). Predictors of hemolytic uremic syndrome in children during a large outbreak of Escherichia coli O157: H7 infections. Pediatrics. 100(1), e12-e12.
Su C & Brandt LJ. (1995) Escherichia coli O157: H7 infection in humans. Annals of internal medicine. 123(9), 698-707.
Bach S, et al. (2002). Transmission and control of Escherichia coli O157: H7-a review. Canadian journal of animal science. 82(4), 475-490.
Slutsker L, et al. (1998). A nationwide case-control study of Escherichia coli O157: H7 infection in the United States. Journal of Infectious Diseases. 177(4), 962-966.
Kiat TS, et al. (2006). Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), towards dengue-2 virus NS3 protease. Bioorganic & medicinal chemistry letters. 16(12), 3337-3340.
Tang SW, et al. (2007). Characterization of flavonoid derivatives from Boesenbergia rotunda (L.). Malaysian Journal of Analytical Sciences. 11(1), 154-159.
Atun S, et al. (2018). Potential bioactive compounds isolated from Boesenbergia rotunda as antioxidant and antimicrobial agents. Pharmacognosy Journal. 10(3).
Eng-Chong T, et al. (2012). Boesenbergia rotunda: from ethnomedicine to drug discovery. Evidence‐Based Complementary and Alternative Medicine. 2012(1), 473637.
Isa N, et al. (2012). In vitro anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A, a chalcone isolated from Boesenbergia rotunda (L.) (fingerroot). Brazilian Journal of Medical and Biological Research. 45, 524-530.
Zainin N, et al. (2013). Antibacterial activity of Boesenbergia rotunda (L.) Mansf. A. extract against Escherichia coli. International Food Research Journal. 20(6), 3319.
Kanjanasirirat P, et al. (2020). High-content screening of Thai medicinal plants reveals Boesenbergia rotunda extract and its component Panduratin A as anti-SARS-CoV-2 agents. Scientific reports. 10(1), 19963.
Rithichai P, et al. (2022). Growth, bioactive compound accumulation and antioxidant activity in rhizomes and storage roots of Boesenbergia rotunda (L.) Mansf. Agriculture and Natural Resources. 56(2), 299–306-299–306.
Kurutas EB, et al. (2015). The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition journal. 15, 1-22.
Tehfe MA, et al. (2013). New chromone based photoinitiators for polymerization reactions under visible light. Polymer Chemistry. 4(15), 4234-4244.
Saeed N, et al. (2012). Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC complementary and alternative medicine. 12, 1-12.
Domig KJ, et al. (2007). Antibiotic susceptibility testing of Bifidobacterium thermophilum and Bifidobacterium pseudolongum strains: Broth microdilution vs. agar disc diffusion assay. International Journal of Food Microbiology. 120(1-2), 191-195.
Nworu C & Esimone, C. (2006) Comparative evaluation of three in vitro techniques in the interaction of ampicillin and ciprofloxacin against Staphylococcus aureus and Escherichia coli. Tropical Journal of Pharmaceutical Research. 5(2), 605-611.
Arslan H, et al. (2020). Risk factors for ciprofloxacin resistance among Escherichia coli strains isolated from community-acquired urinary tract infections in Turkey. Journal of Antimicrobial Chemotherapy. 56(5), 914-918.
Becerra M & Albesa I. (2002). Oxidative stress induced by ciprofloxacin in Staphylococcus aureus. Biochemical and biophysical research communications. 297(4), 1003-1007.
Kleiman M, et al. (2016). Determination of Factors Influencing the Wet Etching of Polydimethylsiloxane Using Tetra-n-butylammonium Fluoride. Macromolecular Chemistry and Physics. 217(2), 284-291.
Adhikari D, et al. (2020). Vasorelaxant effect of Boesenbergia rotunda and its active ingredients on an isolated coronary artery. Plants. 9(12), 1688.
Han C, et al. (2024). Investigation of antimicrobial, antioxidant, and cytotoxic activities of Boesenbergia rotunda rhizome extract. Journal of Current Science and Technology. 14(1), 20-20.
แพรวพรรณ บุญนวม และคณะ. (2564). วารสารวิจัยและพัฒนาระบบสุขภาพ. 14 (3), 152-165.