The study of antioxidant in vitro of longevity Recipe from Pramuan tumrubya Arokayasarn Wat Pa Kut Chanuan Udomphon

Abstract

According to the Arokayasala Medicinal Formula Book, Wat Pa Kut Chanuan Udomporn, the elixir of life is described as a tonic with properties that promote health and potentially extend lifespan. The aim of this study was twofold: 1) to investigate the pharmacological effects, particularly the antioxidant properties, of the elixir of life formula from the Arokayasala Medicinal Formula Book, Wat Pa Kut Chanuan Udomporn, and 2) to provide foundational information for     the development of health products. The elixir of life formula was extracted using a traditional method, with the resulting extract divided into two parts: Part 1, the traditional extract, and Part 2, the extract obtained through freeze drying (solvent evaporation). The total phenolic content and antioxidant activity was assessed using the DPPH assay and FRAP assay. The results indicated that the traditional extract had a total phenolic content of 22.88±1.48 mg GAE/g extract, with an            IC₅₀ value of 57.54±3.23 µg/mL (DPPH assay) and 18.22±0.57 mg Trolox® equivalent/g extract   (FRAP assay). The freeze-dried extract exhibited a total phenolic content of 65±1.62 mg GAE/g extract, with an IC₅₀ value of 20.09±4.86 µg/mL (DPPH assay) and 47.87±4.14 mg Trolox® equivalent/g extract (FRAP assay). These findings suggest that the elixir of life formula exhibits pharmacological activity, particularly in terms of antioxidant properties, with the freeze dry extract showing superior effects. The researchers hope that these results will serve as foundational data for future development of health products based on this formula.