Preliminary Phytochemical Study and Antioxidant Activity of Five Flowers Remedy

Abstract

This research is quantitative study based on quasi-experimental research. The objectives of this study were to 1) study the preliminary phytochemicals of a group of five flowers remedy, namely, Jasminum sambac, Mimusops elengi, Mesua ferrea, Mammea siamensis and Nelumbo nucifera, 2) to further study the antioxidant activity. Phytochemical determination was studied in ethanol extracts of five flowers remedy. Antioxidant activity was studied by DPPH method and determination of total phenolic content was studied by Folin-Ciocalteu method using gallic acid as standard compound. Alkaloids, cardiac glycosides, tannins, flavonoids, terpenoids, and coumarins were found in all five flowers remedy crude extracts. Flowers extract of Nelumbo nucifera and gallic acid were found to have high antioxidant activity at IC₅₀ 0.82 µg/mL and 0.72 µg/mL, respectively. Flowers extract of M. siamensis gave the highest phenolic content at 84.50±0.37 mgGAE.G^-1.