Antioxidant Activities, Total Phenolic and Total Flavonoid Contents of Trisamor Trisutthakula and Tripholsamutthan

Abstract

This study aimed to determine the antioxidant activities, total phenolic and total flavonoid contents of three herb formula in which had been used as food and medication since ancient times. The antioxidant activity was evaluated by DPPH assays, total phenolic content using Folin-Ciocalteau reagent and total flavonoid content. The strongest antioxidant activity was found in the ethanol extract of Tri-smor herb with EC₅₀ = 4.46 ± 0.93 µg/ml, The extract was also showed the highest phenolic contents and flavonoid contents = (18.527 ± 0.169 mg GE/g DW and 3.935 ± 0.102 mg QE/g DW, respectively.). Therefore, the ethanol extract of Tri-smor herbal have the potential of further study and follow-up pharmacological research for the development as active ingredients in medicine.