Analytical Method Development of Chemical Markers in a Thai Traditional Preparation “Feethanuthorawat”

Abstract

Feethanuthorawat preparation is remedy in the Wat Pho Inscriptions containing seven kinds of herbs. An analytical method for chemical marker determination was not developed. The Objective of this study was to quantify contents of marker in the Feethanuthorawat preparation by High Performance Liquid Chromatography HPLC. Qualitative analyses of the Feethanuthorawat preparation was determined using HPLC method. The method was validated on parameters including linearity, precision, accuracy, limit of detection and limit of quantitation. This study, 6-gingerol was simultaneously detected in HPLC chromatogram and identified by comparison with this of the standard compound. Marker (6-gingerol) presented good linearity within the test range (r2 = 0.9994). The relative standard deviation of intraday precision was 1.21%. The relative standard deviation of intraday precision was 1.45%. The recovery of the method was in the range 99.61 to 100.84%. The contents of marker compound in Feethanuthorawat preparation was also quantified by this HPLC method. The limit of detection and the limit of quantitation was 0.13 and 0.39 µg/ml, respectively. The results showed that the content of 6-gingerol was 86.38 mg%. In conclusion, the method is suitable for qualitative analysis of marker in Feethanuthorawat.