Phytochemical Study, Total Phenolic, and Antioxidant Activity of Herbal Extracts for Development of a Hand Treatment Serum

Abstract

This research studied phytochemical screening, total phenolic, and antioxidant activity of Curcuma longa L., Garcinia mangostana L., Centotheca lappacea (L.) Desv., and Phyllanthus emblica L. The study revealed that the phytochemical screening of crude extract detected flavonoids and alkaloid. Furthermore, the total phenolic was studied by Folin-Ciocalteu method, using gallic acid, and later found the total phenolic of C. longa, G. mangotana, C. lappacea, and P. emblica were 481.12 ± 1.00, 399.45 ± 1.15, 355.06 ± 2.30, 41.67.00 mg GAEg^-1 respectively. When analyzed for antioxidant activity by DPPH method, it showed that the crude extract from P. emblica contained the highest antioxidant activity, with an IC₅₀ of 0.62 µg/mL. On the other hand, the crude extract from G. mangostana, C. longa, and C. lappacea indicated different value of IC₅₀ which were 8.41, 22.21, and 97.16, respectively. The herbal extracts were used for preparing a hand treatment serum.