The study of benefit of an additional Ziehl-Neelsen stain for detection of acid-fast bacilli in tissue sections
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Abstract
Objective: This study was aimed to determine whether an additional Ziehl-Neelsen (ZN) stain section could increase the positive rate.
Materials and Methods:
Formalin-fixed paraffin-embedded (FFPE) tissue with positive polymerase chain reaction (PCR)-based detection of M. tuberculosis were retrieved from the Pathology File at King Chulalongkorn Memorial Hospital from 2016 to 2018. The original ZN stain was reviewed, with an additional ZN stain performed.
Results: Of 78 cases with positive PCR, 15 (19.23%) were positive for acid-fast bacilli (AFB) in the original ZN section. Additional 4 cases (19 positive cases in total) were shown to contain AFB in the additional ZN stain section (sensitivity increased from 19.23% to 24.36%).
Conclusion: An additional ZN stain section increased sensitivity (5.13%) for the detection of AFB in tissue sections.
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