Evaluation of Identification and Antimicrobial Susceptibility Testing from Direct Blood Cultures
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Abstract
Backgrounds: Bacterial sepsisis the major cause of death among hospitalized patients. Rapid and accurate laboratory diagnosis of bloodstream infections is important for patient management.
Objectives: To evaluatedthe efficiency of identification and antimicrobial susceptibility testing of bacteria from direct blood culturescompared to standard methods using VITEK®2 compact automation atSurinHospital.
Methods: In this study, only positive samples from culture bottles of total 98 blood samples from patients admitted at Surin Hospital were selected to further evaluate. Each selected blood sample was prepared by centrifugation for direct pellet collection and comparison with the corresponding standard bacterial culture for. The results were analyzed with VITEK® 2 compact for identification and antibiotic susceptibility testing.
Results: The bacterial identification test by direct blood culturecompared with the standard method revealed that 66 samples were Gram-negative bacteria.The sensitivityand 95%CI were 95.5% and 87.3-99.1% respectively. While32 samples were Gram-positive bacteria. The sensitivity and 95%CI were 84.4% and 67.2-94.7%,respectively. The antimicrobial susceptibility test was performed. The Essential agreement (EA) and Categorical agreement (CA) were 98% and 96.5%, respectively, with an error of 3.5% for Gram-negative bacteria. EA and CA of Gram-positive bacteria were 94% with an error rate of 5.6% comparing to the standard method. Theduration time required for identification and susceptibility testing from using direct blood culture was 10-16 hours and the standard method was 34-40 hours. The results found that using direct blood culture spent significant shorter time comparing to that using standard method (p< 0.0001).
Conclusions: The identification and antimicrobial susceptibility test of bacteria from direct blood culture weremore effective and higher sensitivityfor Gram-negative bacteria diagnosis. This method has a reduced sample preparation-to-report time of 10-24hours comparing to the standard method. As a result, doctors are able to diagnose bloodstream infections and provide appropriate treatment for patients more quickly, as well as decrease in patient mortality.
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References
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